OMAL Technology Development
Technology Development
- Beyond Confocal
- Current Technology Development Projects:Inter-relationship of current technology development projects in OMAL that collectively advance mutli-functional microscopy for comprehensive understanding of molecular interactions in living cells.
- Abbreviations:
- PALM: photo-activation localization microscopy
- FRET: Forster resonance energy transfer
- FLIM: fluorescence life-time imaging
- FIDA: fluorescence intensity distribution analysis
- FCS: fluorescence correlation spectroscopy
- ICS: image correlation spectroscopy
- ICCS: image cross correlation spectroscopy
- FRAP: fluorescence recovery after photobleaching
OMAL Current Technologies Overview
- New Technology Development Projects: OMAL participates in a broader imaging program at NCI – Frederick to provide seamless imaging across scales from whole animal imaging to electron microscopy.
OMAL New Technologies Overview
Software Development
Some software tools from OMAL Toolbox
Applications of OMAL Toolbox
- With NCI Collaborators
- Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP (Dr. Tom Misteli)
- Enumeration of PML bodies in cells homozygous for SUMO-1 (Dr. Mike Kuehn)
- Spatial statistical determination that puncta of antisense RNA and P-body foci are co-localized in yeast cells (Dr. David Garfinkel)
- Size, shape and position of centrosomes in migrating cells (Dr. Terry Yamaguchi, planned)
- Utilization of Software distributed via licensing or MTA
- Analysis of changes in the skin epidermis (Unilever Inc.)
- Subnuclear positioning of gene sequences during stem cell differentiation (Professor David Gilbert, Florida State University)
- Measurement of tumors in the mouse colon (ABCC in collaboration with SAIP)
Other Software Developments
- Quantification of Actin Redistribution in Response to Candidate Pharmaceuticals (Dr. John Beutler, Dr. Karlyne Reilly)
- Solid Tumor Image Registration By Hierarchical Decomposition Assisted Automatic Landmarks Detection (Internal Tech Dev)
- Quantification of cells/nuclei from high resolution whole slide scanner (Dr. Karlyne Reilly, Dr. Jake Estes, Dr. Esta Sterneck)
- HTLV-I p8 protein increases viral transmission via induction of tunneling nanotubes (Dr. Genoveffa Franchini)
- HIV-1 Single Virion Analysis: High Efficiency of Genomic RNA Packaging and Heterozygote Formation (Dr. Wei-Shau Hu)
Collaborative Research
OMAL’s collaborative research with NCI and NIH Princial Investigators and also external institutions and corporations have led to significant new technologies:
