Absolute Quantitation of Biologically Important Proteins in Clinical Samples
Most clinical assays are based on the measurement of the absolute quantity of specific molecules. In the Laboratory of Proteomics and Analytical Technologies (LPAT), we have developed solutions that can be customized to measure metabolites or proteins of interest to NCI investigators. Development begins with a request from an NCI investigator for a specific molecule(s) and then LPAT staff investigate whether an assay already exists or a customized solution is required. Once the correct solution is agreed upon, the plan is executed and the completed assay is tested against a series of standards to determine its robustness, accuracy, and precision. LPAT is capable of designing quantitative assays for a wide variety of molecules including metabolites, steroid hormones, lipids, carbohydrates, amino acids, proteins, etc. These assays have been designed for a wide variety of different biological matrices including serum, plasma, urine, and tissue.
Over the past 12 months, LPAT scientists have developed over 12 different assays. These have included assays to validate specific molecules found in metabolomic screens conducted by NCI investigators with Metabolon.