Quantitative Proteomics of Cardiomyopathy in Thin Tissue Sections
An innovative technology for effective proteomic profiling of thin fresh-frozen tissue sections using mass spectrometry (MS) was developed by LPAT scientists in collaboration with Dr. Mark Simpson (CCR) focused on elucidating proteins driving the H-Ras-G12V induced hypertrophic cardiomyopathy in transgenic mice. This proteomic study showed the activation of the Wnt/β-catenin pathway during the pathogenic myocardial hypertrophy, secondary to constitutive H-Ras activation. A number of key proteins were quantified in frozen tissue sections were cross-validated using antibody-based methods such as WB and IHC. The MS-based proteomics approach may be applied to many different tissue types for in vivo molecular profiling and improve the understanding of the pathology driving many other diseases.
Wei BR, Simpson RM, Johann DJ, Dwyer JE, Prieto DA, Kumar M, Ye X, Luke B, Shive HR, Webster JD, Hoover SB, Veenstra TD, Blonder J. Proteomic Profiling of H-Ras-G12V Induced Hypertrophic Cardiomyopathy in Transgenic Mice Using Comparative LC-MS Analysis of Thin Fresh-Frozen Tissue Sections. J Proteome Res. 11(3):1561-70, 2012.