MALDI imaging in combination with LET technology
This study is in collaboration with the Laboratory of Pathology at the Advanced Technology Center in Gaithersburg. They developed the Layered Electrophoretic Transfer technique (LET) and have applied it to tissue analysis. LET technology allows for protein fractionation/enrichment in SDS gel with different concentration without loosing spatial distribution of the species. Combining LET with MALDI imaging can provide information about the intact molecular weight of the species and guide to identification. We are developing a support suitable both for blotting procedure and MALDI process.