Metabolic analysis of cells using the Seahorse Biosciences Flux Analyzer XF-24
The ability to measure changes in cellular metabolism has enabled scientists to begin to connect genomic and proteomic data to physiologic traits of cells in a variety of disease states. For example, malignant transformation of cells is often characterized by aerobic glycolysis, which is the conversion of glucose to lactate in the absence of oxygen. This metabolic shift known as the Warburg effect can result from activation or mutation of oncogenes and tumor suppressors or an adaption to hypoxic conditions. In contrast normal differentiated cells rely on oxidative phosphorylation to generate ATP. These bioenergetic changes associated with malignant transformation are being used to identify new cancer-selective therapeutics.
Seahorse Bioscience has developed a novel platform that measures the oxygen consumption rate and extracellular acidification rate of cells. This provides information about oxidative phosphorylation and glycolysis simultaneously. PCL has a Seahorse Biosciences Metabolic Flux Analyzer XF-24 that enables these measurements to be made in a 24-well plate format. PCL is in the process of developing a series of assays that will be used to train new users. Once users have been trained they will have walk-up access of the instrument.