PEL Services
The Protein Expression Laboratory (PEL) offers a variety of core services. To order services, please submit an electronic request via CSAS. For additional information on how to use the on-line accession system please click here. To discuss the project scope and design, please contact the individual group leader, or Dominic Esposito.
Clone Optimization
- Custom DNA cloning
- Subcloning
- Plasmid DNA preparation
- Site-directed mutagenesis
- Custom vector construction
- Gene optimization
- shRNA/miRNA expression and reporter constructs
- Consulting
Microbial Expression
- Transformations of bacteria and yeast strains
- Colony screening for expression optimization in yeast
- Expression screening in bacteria to maximize recombinant protein quantity and quality
- Batch, batch-fed, or continuous fermentation in multiple yeast and bacteria strains (2ml to 60L)
- TFF Concentration, Buffer Exchange, and Ultrafiltration
- Cell lysate preparation and clarification
- Analysis: SDS-PAGE, Western Blots, Dot Blots, etc.
Protein Purification
- Micro-scale purification screening and optimization
- Scale-up from micrograms to grams
- Endotoxin testing and removal
- Customized Protein Purification
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Eukaryotic Expression
- Expression of recombinant proteins using the baculovirus expression vector system (BEVS)
- Monoclonal antibody production
- Recombinant protein expression in mammalian cells
Cell Free Expression
- Using normal amino acids
- Using stable isotopes
- Scalable reaction from 50ul to 5ml (yield up to mg’s of protein depending on protein)
Viral Technology Group
- Lentivirus packaging, titration (FACS, qPCR),
- Lentivirus promoter panel
- Stock control viruses (promoter, +/- reporter gene, +/- selection marker)
- SBI shRNA library screening support
- Adenovirus production from adenoviral DNA construct or amplification from purified adenovirus
- Titration of adenovirus by Clontech Rapid Titer Assay, traditional plaque assay or fluorescent marker (FACS)
Molecular Detection
- Cell Line Quality Control, including mycoplasma testing, STR analysis, and human pathogen screen
- Validated qPCR assays, custom assays, or SABiosciences PCR arrays
- Serology assays to detect viral antigens/antibodies (custom), multiplexing, or testing using commercially available assays
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