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High-throughput system for expression testing in E. coli using different mediums and temperatures

The main goal of this technology development effort in the Protein Expression Laboratory (PEL) is to develop a high-throughput system using small blocks capable of growing 24 individual cultures.  Each block can used to grow a culture in a specific medium at a specific temperature.  This technology will reduce material and labor costs needed for larger cultures while producing materials suitable for “purify first” technology (see figure comparing eleven proteins expressed by IPTG or lactose induction).

Page last updated April 27, 2011 @ 4:08 pm