On Demand Protein Microarray
Protein Microarrays are becoming an indispensable tool to study interactions with drugs, antibodies, other proteins, lipids, nucleic acids, etc. The Protein Expression Laboratory (PEL) has developed a novel microarray system in which immobilized expression plasmid DNA molecules are transcribed and translated into protein in situ, and the newly expressed proteins are captured at the site of their expression on a slide or a chip. Expression, purification and capturing of proteins are all done in the slide. This microarray should be very useful for various applications to study and validate protein-protein interactions, antibody screening, drug screening, etc.
The advantages of this novel technology over the current technology:
- DNA microarray easily converted to protein on the microarray by in vitro transcription/translation
- Protein expression, purification and capture occur on the microarray
- Content highly customizable
- Low cost production
Plasmids spotted on the microarray encode proteins of interest fused to the Tus protein, that after synthesis, bind to a Ter sequence encoded elsewhere on the plasmids. For more details about the technology, see:
Chatterjee DK, Sitaraman K, Baptista C, Hill T, Hartley J, Munroe D: In situ Protein Array Technology (ISPAT)—A simple high throughput protein microarray system. PLoS ONE, 3(9): e3265, 2008.