TRANSFORMING GROWTH FACTOR b-1(rDNA HUMAN)
- AMPOULE CONTENTS
Each ampoule contains the residue after freeze-drying of 1 ml of phosphate buffered
saline containing: Nominally, 160 ng TGF b-1 0.6%; human serum albumin.
The ampoules do not contain bacteriostat.
The assigned potency is 3,000 reference units (RU) per ampoule.
The preparation contains material of human origin which has been tested and found
negative for HBsAg and HIV antibody. However, as with all materials of
biological origin, this preparation should be regarded as potentially hazardous
to health. It should be used and discarded according to your own laboratory's safety
procedures. Such safety procedures probably will include the wearing of protective
gloves and avoiding the generation of aerosols. Care should be exercised in opening
ampoules or vials, to avoid cuts. This preparation is not for administration to
- USE OF AMPOULED MATERIAL
For all practical purposes, each ampoule contain the same quantity of the substances
listed above. Dissolve the total contents of the ampoule with 0.5 ml of sterile
distilled water. Rinse the ampoule with 0.4 ml of distilled water and make up the
total volume to 1 ml with distilled water. This solution should be now at a concentration
of 3,000 units/ml. Use carrier protein where extensive dilution is required. No
attempt should be made to weigh out any portion of tex freeze-dried material.
Unopened ampoules should be stored at -20oC. For economy use, it is recommended
that the solution be sub-divided into several small aliquots an stored at -40oC
In all publications in which this preparation is used as an assay calibrant, it
is important that the title of the preparation and ampoule code be cited correctly.
This reagent is available in small amounts (approximately 0.16 microgram/sample)
for use in the calibration of in vitro bioassays and in-house standards only and
are not to be used for experimental purposes. Reference reagents are available to
investigators with peer-reviewed support, at institutions or at commercial establishments
and are limited to one sample of each reagent per year.