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A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology

  1. Author:
    Gao, Y. D.
    Majumdar, D.
    Jovanovic, B.
    Shaifer, C.
    Lin, P. C.
    Zijlstra, A.
    Webb, D. J.
    Li, D. Y.

  2. Author Address

    [Gao, YD; Li, DY] Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA [Majumdar, D; Webb, DJ] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA [Majumdar, D; Webb, DJ] Vanderbilt Univ, Vanderbilt Kennedy Ctr Res Human Dev, Nashville, TN 37235 USA [Jovanovic, B; Webb, DJ] Vanderbilt Univ, Dept Canc Biol, Nashville, TN 37235 USA [Shaifer, C] Meharry Med Coll, Dept Biochem & Canc Biol, Nashville, TN 37208 USA [Lin, PC] NCI, Ctr Canc Res, Frederick, MD 21702 USA [Zijlstra, A] Vanderbilt Univ, Dept Pathol, Nashville, TN 37235 USA;Lin, PCLi, DY (reprint author), Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA;deyu.li@vanderbilt.edu
    1. Year: 2011
    2. Date: Jun
  2. Journal: Biomedical Microdevices
    1. 13
    2. 3
    3. Pages: 539-548
  4. Type of Article: Article
  5. ISSN: 1387-2176
  1. Abstract:

    A versatile microfluidic platform allowing co-culture of multiple cell populations in close proximity with separate control of their microenvironments would be extremely valuable for many biological applications. Here, we report a simple and compact microfluidic platform that has these desirable features and allows for real-time, live-cell imaging of cell-cell interactions. Using a pneumatically/hydraulically controlled poly(dimethylsiloxane) (PDMS) valve barrier, distinct cell types can be cultured in side-by-side microfluidic chambers with their optimum culture media and treated separately without affecting the other cell population. The platform is capable of both two-dimensional and three-dimensional cell co-culture and through variations of the valve barrier design, the platform allows for cell-cell interactions through either direct cell contact or soluble factors alone. The platform has been used to perform dynamic imaging of synapse formation in hippocampal neurons by separate transfection of two groups of neurons with fluorescent pre- and post-synaptic protein markers. In addition, cross-migration of 4T1 tumor cells and endothelial cells has been studied under normoxic and hypoxic conditions, which revealed different migration patterns, suggesting the importance of the microenvironments in cell-cell interactions and biological activities.

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External Sources

  1. View Full Text
  2. DOI: 10.1007/s10544-011-9523-9
  3. View record in Web of Science®
  4. WOS: 000290099600013

Library Notes

  1. Fiscal Year: FY2010-2011
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