Skip NavigationSkip to Content
Return Return to Search Results

Islet1-mediated activation of the beta-catenin pathway is necessary for hindlimb initiation in mice

  1. Author:
    Kawakami, Y.
    Marti, M.
    Kawakami, H.
    Itou, J.
    Quach, T.
    Johnson, A.
    Sahara, S.
    O'Leary, D. D. M.
    Nakagawa, Y.
    Lewandoski, M.
    Pfaff, S.
    Evans, S. M.
    Belmonte, J. C. I.

  2. Author Address

    [Kawakami, Y; Kawakami, H; Pfaff, S; Belmonte, JCI] Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA. [Kawakami, Y; Kawakami, H; Itou, J; Quach, T; Johnson, A] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA. [Kawakami, Y; Kawakami, H; Itou, J; Nakagawa, Y] Univ Minnesota, Stem Cell Inst, Minneapolis, MN 55455 USA. [Kawakami, Y; Nakagawa, Y] Univ Minnesota, Ctr Dev Biol, Minneapolis, MN 55455 USA. [Kawakami, Y] Univ Minnesota, Masonic Canc Ctr, Minneapolis, MN 55455 USA. [Marti, M; Belmonte, JCI] Ctr Regenerat Med Barcelona, Barcelona 08003, Spain. [Sahara, S; O'Leary, DDM] Salk Inst Biol Studies, Molecular Neurosci Lab, La Jolla, CA 92037 USA. [Nakagawa, Y] Univ Minnesota, Dept Neurosci, Minneapolis, MN 55455 USA. [Lewandoski, M] NCI, Canc & Dev Biol Lab, Frederick, MD 21702 USA. [Evans, SM] Univ Calif San Diego, Skaggs Sch Pharm, La Jolla, CA 92093 USA. [Evans, SM] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA.;Kawakami, Y (reprint author), Salk Inst Biol Studies, Gene Express Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA;kawak005@umn.edu belmonte@salk.edu
    1. Year: 2011
    2. Date: Oct
  2. Journal: Development
    1. 138
    2. 20
    3. Pages: 4465-4473
  4. Type of Article: Article
  5. ISSN: 0950-1991
  1. Abstract:

    The transcriptional basis of vertebrate limb initiation, which is a well-studied system for the initiation of organogenesis, remains elusive. Specifically, involvement of the beta-catenin pathway in limb initiation, as well as its role in hindlimb-specific transcriptional regulation, are under debate. Here, we show that the beta-catenin pathway is active in the limb-forming area in mouse embryos. Furthermore, conditional inactivation of beta-catenin as well as Islet1, a hindlimb-specific factor, in the lateral plate mesoderm results in a failure to induce hindlimb outgrowth. We further show that Islet1 is required for the nuclear accumulation of. catenin and hence for activation of the beta-catenin pathway, and that the beta-catenin pathway maintains Islet1 expression. These two factors influence each other and function upstream of active proliferation of hindlimb progenitors in the lateral plate mesoderm and the expression of a common factor, Fgf10. Our data demonstrate that Islet1 and beta-catenin regulate outgrowth and Fgf10-Fgf8 feedback loop formation during vertebrate hindlimb initiation. Our study identifies Islet1 as a hindlimb-specific transcriptional regulator of initiation, and clarifies the controversy regarding the requirement of beta-catenin for limb initiation.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1242/dev.065359
  3. View record in Web of Science®
  4. WOS: 000295085600014

Library Notes

  1. Fiscal Year: FY2011-2012
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel