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Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System

  1. Author:
    Gillette,Bill
    Frank,Peter
    Perkins,Shelley
    Drew,Matt
    Grose,Carissa
    Esposito,Dom

  2. Author Address

    Protein Expression Laboratory, NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Protein Expression Laboratory, NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. dom.esposito@nih.gov.,
    1. Year: 2019
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 2009
    2. Pages: 259-277
  4. Type of Article: Book Chapter
  5. ISSN: 978-1-4939-9531-8
  1. Abstract:

    Protein prenylation is a common posttranslational modification that enhances the ability of proteins to interact with membrane components within the cell. In many cases, these prenylated proteins are involved in important human diseases, including aging-related disorders and cancer. To effectively study these proteins or develop therapeutics, large quantities of properly modified proteins are required. Historically, production of fully modified farnesylated and methylated proteins at high yield has been challenging. Recently, an engineered insect cell system which is capable of producing authentically modified KRAS protein was used to generate material for structural studies and assay development. Here we describe protocols for extending this work to other farnesylated and methylated substrates.

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External Sources

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  2. DOI: 10.1007/978-1-4939-9532-5_20
  3. PMID: 31152410
  4. View record in Web of Science®
  5. WOS: 000487844400021

Library Notes

  1. Fiscal Year: FY2018-2019
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