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Human Plasma-like Medium Improves T Lymphocyte Activation

  1. Author:
    Leney-Greene, Michael A
    Boddapati,Arun Kumar
    Su, Helen C
    Cantor, Jason R
    Lenardo, Michael J

  2. Author Address

    Molecular Development of the Immune System Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Immunology Graduate Group, Biomedical Graduate Studies, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA., NIAID Collaborative Bioinformatics Resource (NCBR), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Immunology Graduate Group, Biomedical Graduate Studies, University of Pennsylvania, Philadelphia, PA 19104, USA; Human Immunological Diseases Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Morgridge Institute for Research, 330 North Orchard Street, Madison, WI 53715, USA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA; Carbone Cancer Center, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI 53705, USA., Molecular Development of the Immune System Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Immunology Graduate Group, Biomedical Graduate Studies, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: michael.lenardo@nih.gov.,
    1. Year: 2020
    2. Date: JAN 24
    3. Epub Date: 2019 12 11
  2. Journal: iScience
    1. 23
    2. 1
    3. Pages: 100759
  4. Type of Article: Article
  5. Article Number: 100759
  6. ISSN: 2589-0042
  1. Abstract:

    T lymphocytes are critical for effective immunity, and the ability to study their behavior in vitro can facilitate major insights into their development, function, and fate. However, the composition of human plasma differs from conventional media, and we hypothesized that such differences could impact immune cell physiology. Here, we showed that relative to the medium typically used to culture lymphocytes (RPMI), a physiologic medium (human plasma-like medium; HPLM) induced markedly different transcriptional responses in human primary T cells and in addition, improved their activation upon antigen stimulation. We found that this medium-dependent effect on T cell activation is linked to Ca2+, which is six-fold higher in HPLM than in RPMI. Thus, a medium that more closely resembles human plasma has striking effects on T cell biology, further demonstrates that medium composition can profoundly affect experimental results, and broadly suggests that physiologic media may offer a valuable way to study cultured immune cells. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.isci.2019.100759
  3. PMID: 31887663
  4. PMCID: PMC6941860
  5. View record in Web of Science®
  6. WOS: 000508685200030
  7. PII : S2589-0042(19)30504-8

Library Notes

  1. Fiscal Year: FY2019-2020
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