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Enterotoxigenic Bacteroides fragilis infection exacerbates tumorigenesis in AOM/DSS mouse model

  1. Author:
    Hwang, Soonjae
    Lee, Chang Gun
    Jo, Minjeong
    Park, Chan Oh
    Gwon, Sun-Yeong
    Hwang, Samnoh
    Yi, Hye Chin
    Lee, So-Yeon
    Eom, Yong-Bin
    Karim,Baktiar
    Rhee, Ki-Jong

  2. Author Address

    Yonsei Univ Wonju, Coll Hlth Sci, Dept Biomed Lab Sci, Wonju 26493, Gangwon Do, South Korea.Yonsei Univ, Cell Therapy & Tissue Engn Ctr, Wonju Coll Med, Wonju 26426, South Korea.Soonchunhyang Univ, Coll Med Sci, Dept Med Sci, Asan 31538, Chungnam, South Korea.Soonchunhyang Univ, Coll Med Sci, Dept Biomed Lab Sci, Asan 31538, Chungnam, South Korea.Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA.
    1. Year: 2020
    2. Epub Date: 2020 01 01
  2. Journal: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
  3. IVYSPRING INT PUBL,
    1. 17
    2. 2
    3. Pages: 145-152
  5. Type of Article: Article
  6. ISSN: 1449-1907
  1. Abstract:

    The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice.

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External Sources

  1. View Full Text
  2. DOI: 10.7150/ijms.38371
  3. PMID: 32038097
  4. PMCID: PMC6990882
  5. View record in Web of Science®
  6. WOS: 000508259400001

Library Notes

  1. Fiscal Year: FY2019-2020
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