Changes in the core endophytic mycobiome of carrot taproots in response to crop management and genotype

Author:

Abdelrazek, Sahar

Choudhari,Sulbha

Thimmapuram, Jyothi

Simon, Philipp

Colley, Micaela

Mengiste, Tesfaye

Hoagland, Lori
Author Address

Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA., Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Bioinformatics Core, Purdue University, West Lafayette, IN, USA., USDA-ARS Agriculture Research Service, Madison, WI, USA., Organic Seed Alliance, Port Townsend, Washington, USA., Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, USA., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA. lhoaglan@purdue.edu.,

Abstract:

Fungal endophytes can influence production and post-harvest challenges in carrot, though the identity of these microbes as well as factors affecting their composition have not yet been determined, which prevents growers from managing these organisms to improve crop performance. Consequently, we characterized the endophytic mycobiome in the taproots of three carrot genotypes that vary in resistance to two pathogens grown in a trial comparing organic and conventional crop management using Illumina sequencing of the internal transcribed spacer (ITS) gene. A total of 1,480 individual operational taxonomic units (OTUs) were identified. Most were consistent across samples, indicating that they are part of a core mycobiome, though crop management influenced richness and diversity, likely in response to differences in soil properties. There were also differences in individual OTUs among genotypes and the nematode resistant genotype was most responsive to management system indicating that it has greater control over its endophytic mycobiome, which could potentially play a role in resistance. Members of the Ascomycota were most dominant, though the exact function of most taxa remains unclear. Future studies aimed at overcoming difficulties associated with isolating fungal endophytes are needed to identify these microbes at the species level and elucidate their specific functional roles.

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