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Tumor-associated macrophages derived from cancer stem cells

  1. Author:
    Osman, Amira
    Oze, Miharu
    Afify, Said M
    Hassan, Ghmkin
    El-Ghlban, Samah
    Nawara, Hend M
    Fu, Xiaoying
    Zahra, Maram Hussein
    Seno, Akimasa
    Winer, Ira
    Salomon,David
    Seno, Masaharu

  2. Author Address

    Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Department of Histology, Faculty of Medicine, Kafrelsheikh University, 32511, Egypt. Electronic address: mero.osman@med.kfs.edu.eg., Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan. Electronic address: px934zug@s.okayama-u.ac.jp., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Division of Biochemistry, Chemistry Department, Faculty of Science, Menoufia University, Shebin El Koum-Menoufia, 32511, Egypt. Electronic address: saidafify@s.okayama-u.ac.jp., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Department of Microbiology and Biochemistry, Faculty of Pharmacy, Damascus University, Damascus, 10769, Syria. Electronic address: pthz2c4o@s.okayama-u.ac.jp., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Division of Biochemistry, Chemistry Department, Faculty of Science, Menoufia University, Shebin El Koum-Menoufia, 32511, Egypt. Electronic address: phcm6dy1@okayama-u.ac.jp., Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan. Electronic address: pnt32hvi@s.okayama-u.ac.jp., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Department of Pathology, Tianjin University of Traditional Chinese Medicine, Tianjin, P.R. China, 300193. Electronic address: fuxiaoyingtj@163.com., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan. Electronic address: maram@okayama-u.ac.jp., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Okayama University Research Laboratory of Stem Cell Engineering in Detroit, IBio, Wayne State University, MI, 48202, USA. Electronic address: aseno@okayama-u.ac.jp., Department of Obstetrics & Gynecology, School of Medicine, Wayne State University, MI, 48202, USA. Electronic address: iwiner@med.wayne.edu., Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, MD, 21702, USA. Electronic address: salomond@mail.nih.gov., Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, 700-8530, Japan; Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan; Okayama University Research Laboratory of Stem Cell Engineering in Detroit, IBio, Wayne State University, MI, 48202, USA. Electronic address: mseno@okayama-u.ac.jp.,
    1. Year: 2020
    2. Date: Dec
    3. Epub Date: 2020 09 26
  2. Journal: Acta histochemica
    1. 122
    2. 8
    3. Pages: 151628
  4. Type of Article: Article
  5. Article Number: 151628
  6. ISSN: 0065-1281
  1. Abstract:

    Macrophages are the most abundant immune cells in the microenvironment of solid tumors. The present study displayed histological and immunohistochemical analyses of a malignant tumor model developed from cancer stem cells (CSCs) converted from human induced pluripotent stem cells (hiPSCs) in a cancer microenvironment prepared from the conditioned medium (CM) of a pancreatic cancer cell line. We focused on the localization and the origin of tumor-associated macrophages (TAMs), To the best of our knowledge this may be the first study to suggest the potential differentiation of CSCs to TAMs. hiPSCs were converted into CSCs in the presence of CM from PK8 cells. CSCs were then transplanted in vivo and formed primary tumors. Primary cultures for these tumors were serially transplanted again to obtain secondary tumors. Secondary tumors exhibited histopathological features of malignancy. Cells derived from tumors maintained the expression of endogenous stemness markers and pancreatic CSCs markers. Simultaneously, high immunoreactivity to anti-mouse CD68, anti-human CD68, CD206 and CD11b antibodies were detected revealing that the tumor tissue derived from CSCs was enriched for macrophages which can originate from both human and mouse cells. The model of CSCs highlighted the possibility of CSCs to differentiate into TAMs. Copyright © 2020 Elsevier GmbH. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.acthis.2020.151628
  3. PMID: 32992123
  4. View record in Web of Science®
  5. WOS: 000593955400005
  6. PII : S0065-1281(20)30127-6

Library Notes

  1. Fiscal Year: FY2020-2021
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