Skip NavigationSkip to Content
Return Return to Search Results

Origin of rebound virus in chronically SIV-infected Rhesus monkeys following treatment discontinuation

  1. Author:
    Liu, Po-Ting [ORCID]
    Keele,Brandon
    Abbink, Peter [ORCID]
    Mercado, Noe B [ORCID]
    Liu, Jinyan
    Bondzie, Esther A
    Chandrashekar, Abishek [ORCID]
    Borducchi, Erica N
    Hesselgesser, Joseph
    Mish, Michael
    Chin, Gregory
    Bekerman, Elena
    Geleziunas, Romas
    Barouch, Dan H [ORCID]

  2. Author Address

    Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Gilead Sciences, Foster City, CA, USA., Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. dbarouch@bidmc.harvard.edu., Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA, USA. dbarouch@bidmc.harvard.edu.,
    1. Year: 2020
    2. Date: 10 27
    3. Epub Date: 2020 10 27
  2. Journal: Nature communications
    1. 11
    2. 1
    3. Pages: 5412
  4. Type of Article: Article
  5. Article Number: 5412
  6. ISSN: 2041-1723
  1. Abstract:

    Viral rebound following antiretroviral therapy (ART) discontinuation in HIV-1-infected individuals is believed to originate from a small pool of CD4+ T cells harboring replication-competent provirus. However, the origin and nature of the rebound virus has remained unclear. Recent studies have suggested that rebound virus does not originate directly from individual latent proviruses but rather from recombination events involving multiple proviruses. Here we evaluate the origin of rebound virus in 16 ART-suppressed, chronically SIV-infected rhesus monkeys following ART discontinuation. We sequence viral RNA and viral DNA in these animals prior to ART initiation, during ART suppression, and following viral rebound, and we compare rebound viral RNA after ART discontinuation with near full-length viral DNA from peripheral blood and lymph node mononuclear cells (PBMC and LNMC) during ART suppression. Sequences of initial rebound viruses closely match viral DNA sequences in PBMC and LNMC during ART suppression. Recombinant viruses are rare in the initial rebound virus populations but arise quickly within 2-4 weeks after viral rebound. These data suggest that intact proviral DNA in PBMC and LNMC during ART suppression is likely the direct origin of viral rebound in chronically SIV-infected rhesus monkeys following ART discontinuation.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1038/s41467-020-19254-2
  3. PMID: 33110078
  4. View record in Web of Science®
  5. WOS: 000594622700002
  6. PII : 10.1038/s41467-020-19254-2

Library Notes

  1. Fiscal Year: FY2020-2021
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel