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The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization

  1. Author:
    Karger, Amy B [ORCID]
    Brien, James D
    Christen,Jayne
    Dhakal, Santosh [ORCID]
    Kemp,Troy
    Klein, Sabra L [ORCID]
    Pinto,Ligia [ORCID]
    Premkumar, Lakshmanane
    Roback, John D
    Binder, Raquel A
    Boehme, Karl W [ORCID]
    Boppana, Suresh
    Cordon-Cardo, Carlos
    Crawford, James M
    Daiss, John L [ORCID]
    Dupuis, Alan P
    Espino, Ana M [ORCID]
    Firpo-Betancourt, Adolfo
    Forconi, Catherine
    Forrest, J Craig
    Girardin, Roxie C
    Granger, Douglas A
    Granger, Steve W
    Haddad, Natalie S
    Heaney, Christopher D [ORCID]
    Hunt, Danielle T
    Kennedy, Joshua L
    King, Christopher L
    Krammer, Florian [ORCID]
    Kruczynski, Kate
    LaBaer, Joshua
    Lee, F Eun-Hyung
    Lee, William T [ORCID]
    Liu, Shan-Lu
    Lozanski, Gerard
    Lucas, Todd
    Mendu, Damodara Rao
    Moormann, Ann M [ORCID]
    Murugan, Vel
    Okoye, Nkemakonam C
    Pantoja, Petraleigh
    Payne, Anne F
    Park, Jin
    Pinninti, Swetha
    Pinto, Amelia K [ORCID]
    Pisanic, Nora
    Qiu, Ji
    Sariol, Carlos A
    Simon, Viviana
    Song, Lusheng
    Steffen, Tara L
    Stone, E Taylor
    Styer, Linda M [ORCID]
    Suthar, Mehul S
    Thomas, Stefani N [ORCID]
    Thyagarajan, Bharat
    Wajnberg, Ania
    Yates, Jennifer L
    Sobhani, Kimia

  2. Author Address

    Department of Laboratory Medicine and Pathology, University of Minnesotagrid.17635.36, Minneapolis, Minnesota, USA., Department of Molecular Microbiology & Immunology, Saint Louis Universitygrid.262962.b, St. Louis, Missouri, USA., Frederick National Laboratory for Cancer Researchgrid.418021.e, Frederick, Maryland, USA., W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA., Department of Pathology and Laboratory Medicine, grid.471395.dgrid.189967.8Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA., Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Department of Microbiology & Immunology, University of Arkansas for Medical Sciencesgrid.241054.6, Little Rock, Arkansas, USA., Department of Pediatrics, University of Alabama at Birminghamgrid.265892.2, Birmingham, Alabama, USA., Department of Microbiology, University of Alabama at Birminghamgrid.265892.2, Birmingham, Alabama, USA., Department of Pathology, Icahn School of Medicine at Mount Sinaigrid.59734.3c, New York, New York, USA., Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA., MicroB-plex, Inc., Atlanta, Georgia, USA., Wadsworth Centergrid.465543.5, New York State Department of Health, Albany, New York, USA., Department of Microbiology and Medical Zoology, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico, USA., Salimetrics, LLC, Carlsbad, California, USA., Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, grid.471395.dgrid.189967.Emory University, Atlanta, Georgia, USA., Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Department of Pediatrics, University of Arkansas for Medical Sciencesgrid.241054.6, Little Rock, Arkansas, USA., Department of Internal Medicine, University of Arkansas for Medical Sciencesgrid.241054.6, Little Rock, Arkansas, USA., Arkansas Children 39;s Research Institute, Little Rock, Arkansas, USA., Department of Pathology, Case Western Reserve School of Medicine, Cleveland, Ohio, USA., Department of Microbiology, Icahn School of Medicine at Mount Sinaigrid.59734.3c, New York, New York, USA., Virginia G. Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona, USA., Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, USA., Center for Retrovirus Research, The Ohio State Universitygrid.261331.4, Columbus, Ohio, USA., Department of Veterinary Biosciences, The Ohio State Universitygrid.261331.4, Columbus, Ohio, USA., Department of Microbial Infection and Immunity, The Ohio State Universitygrid.261331.4, Columbus, Ohio, USA., Viruses and Emerging Pathogens Program, Infectious Disease Institute, The Ohio State Universitygrid.261331.4, Columbus, Ohio, USA., Department of Pathology, The Ohio State Universitygrid.261331.4 Medical Center, Columbus, Ohio, USA., Division of Public Health, College of Human Medicine, Michigan State University, East Lansing, Michigan, USA., Department of Epidemiology, College of Human Medicine, Michigan State University, East Lansing, Michigan, USA., Unit of Comparative Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico, USA., Department of Internal Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico, USA., Center for Childhood Infections and Vaccines of Children 39;s Healthcare of Atlanta, Department of Pediatrics, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA., Department of Microbiology and Immunology, grid.471395.dgrid.189967.8Emory University, Atlanta, Georgia, USA., Emory Vaccine Center, grid.471395.dgrid.189967.8Emory University, Atlanta, Georgia, USA., Yerkes National Primate Research Center, Atlanta, Georgia, USA., Department of Medicine, Icahn School of Medicine at Mount Sinaigrid.59734.3c, New York, New York, USA., Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Centergrid.50956.3f, Los Angeles, California, USA.,
    1. Year: 2022
    2. Date: Jun 15
    3. Epub Date: 2022 06 15
  2. Journal: mSphere
    1. Pages: e0019322
  4. Type of Article: Article
  5. Article Number: e0019322
  1. Abstract:

    In October 2020, the National Cancer Institute (NCI) Serological Sciences Network (SeroNet) was established to study the immune response to COVID-19, and "to develop, validate, improve, and implement serological testing and associated technologies" (https://www.cancer.gov/research/key-initiatives/covid-19/coronavirus-research-initiatives/serological-sciences-network). SeroNet is comprised of 25 participating research institutions partnering with the Frederick National Laboratory for Cancer Research (FNLCR) and the SeroNet Coordinating Center. Since its inception, SeroNet has supported collaborative development and sharing of COVID-19 serological assay procedures and has set forth plans for assay harmonization. To facilitate collaboration and procedure sharing, a detailed survey was sent to collate comprehensive assay details and performance metrics on COVID-19 serological assays within SeroNet. In addition, FNLCR established a protocol to calibrate SeroNet serological assays to reference standards, such as the U.S. severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology standard reference material and first WHO international standard (IS) for anti-SARS-CoV-2 immunoglobulin (20/136), to facilitate harmonization of assay reporting units and cross-comparison of study data. SeroNet institutions reported development of a total of 27 enzyme-linked immunosorbent assay (ELISA) methods, 13 multiplex assays, and 9 neutralization assays and use of 12 different commercial serological methods. FNLCR developed a standardized protocol for SeroNet institutions to calibrate these diverse serological assays to reference standards. In conclusion, SeroNet institutions have established a diverse array of COVID-19 serological assays to study the immune response to SARS-CoV-2 and vaccines. Calibration of SeroNet serological assays to harmonize results reporting will facilitate future pooled data analyses and study cross-comparisons. IMPORTANCE SeroNet institutions have developed or implemented 61 diverse COVID-19 serological assays and are collaboratively working to harmonize these assays using reference materials to establish standardized reporting units. This will facilitate clinical interpretation of serology results and cross-comparison of research data.

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  2. DOI: 10.1128/msphere.00193-22
  3. PMID: 35703544

Library Notes

  1. Fiscal Year: FY2021-2022
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