Skip NavigationSkip to Content

Effect of peritoneal fluid from women with endometriosis on implantation in the mouse model

  1. Author:
    Illera, M. J.
    Juan, L. W.
    Stewart, C. L.
    Cullinan, E.
    Ruman, J.
    Lessey, B. A.
  2. Author Address

    Lessey BA Univ N Carolina, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil CB 7570,MacNider Bldg Chapel Hill, NC 27599 USA Univ N Carolina, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil Chapel Hill, NC 27599 USA Univ Complutense, Sch Vet Med, Dept Physiol E-28040 Madrid Spain NCI, Lab Canc & Dev Biol Frederick, MD 21701 USA Lexicon Genet Inc The Woodlands, TX USA
    1. Year: 2000
  1. Journal: Fertility and Sterility
    1. 74
    2. 1
    3. Pages: 41-48
  2. Type of Article: Article
  1. Abstract:

    Objective: To investigate the potential role of peritoneal fluid (PF) from women with or without endometriosis in implantation in mice with use of the delayed implantation model. Design: A murine experimental model with markers of uterine receptivity and prospective comparison of the effects of human PF on implantation. Setting: Academic university and hospital program. Intervention(s): PF collected from women with and without endometriosis was injected intraperitoneally into recently mated mice. Main Outcome Measure(s): Implantation sites were counted in treated and untreated animals, and the alpha v beta 3 integrin was measured in the pregnant mouse uterus by immunohistochemistry with in situ hybridization. Leukemia inhibitory factor and the beta 3 subunit of alpha v beta 3 were measured by Northern blot during early pregnancy and after injections of PF. Result(s): Animals receiving PF from infertile women with endometriosis had a reduction in the number of implantation sites compared with animals that received PF from fertile women or from patients with recently treated endometriosis. In the mouse, expression of alpha v beta 3 and leukemia inhibitory factor peaked at the time of implantation and was reduced by injections of human PF from infertile patients with endometriosis. Conclusion(s): Leukemia inhibitory factor and alpha v beta 3 are coexpressed at the time of implantation in the mouse. PF from women with endometriosis has a detrimental effect on embryo implantation, perhaps by adversely affecting uterine receptivity. (Fertil Steril(R) 2000;74:41-8. (C) 2000 by American Society for Reproductive Medicin). [References: 66]

    See More

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel