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Immunopathological and microbial signatures of inflammatory bowel disease in partial RAG deficiency

  1. Author:
    Castagnoli, Riccardo [ORCID]
    Pala, Francesca [ORCID]
    Subramanian, Poorani [ORCID]
    Oguz, Cihan [ORCID]
    Schwarz, Benjamin [ORCID]
    Lim, Ai Ing [ORCID]
    Burns, Andrew S [ORCID]
    Fontana, Elena [ORCID]
    Bosticardo, Marita [ORCID]
    Corsino, Cristina [ORCID]
    Angelova, Angelina [ORCID]
    Delmonte, Ottavia M [ORCID]
    Kenney, Heather [ORCID]
    Riley, Deanna [ORCID]
    Smith, Grace [ORCID]
    Ott de Bruin, Lisa [ORCID]
    Oikonomou, Vasileios [ORCID]
    Dos Santos Dias, Lucas [ORCID]
    Fink,Danielle [ORCID]
    Bohrnsen, Eric [ORCID]
    Kimzey, Cole D [ORCID]
    Marseglia, Gian Luigi [ORCID]
    Alva-Lozada, Guisela [ORCID]
    Bergerson, Jenna R E [ORCID]
    Brett, Ana [ORCID]
    Brigatti, Karlla W [ORCID]
    Dimitrova, Dimana [ORCID]
    Dutmer, Cullen M [ORCID]
    Freeman, Alexandra F [ORCID]
    Ale, Hanadys [ORCID]
    Holland, Steven M [ORCID]
    Licciardi, Francesco [ORCID]
    Pasic, Srdjan [ORCID]
    Poskitt, Laura E [ORCID]
    Potts, David E [ORCID]
    Dasso, Joseph F [ORCID]
    Sharapova, Svetlana O [ORCID]
    Strauss, Kevin A [ORCID]
    Ward, Brant R [ORCID]
    Yilmaz, Melis [ORCID]
    Kuhns,Douglas [ORCID]
    Lionakis, Michail S [ORCID]
    Daley, Stephen R [ORCID]
    Kong, Heidi H [ORCID]
    Segre, Julia A [ORCID]
    Villa, Anna [ORCID]
    Pittaluga, Stefania [ORCID]
    Walter, Jolan E
    Vujkovic-Cvijin, Ivan [ORCID]
    Belkaid, Yasmine [ORCID]
    Notarangelo, Luigi D [ORCID]

  2. Author Address

    Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Bioinformatics and Computational Biosciences Branch, Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD, USA., Integrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, MT, USA., Metaorganism Immunity Section, Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., NIAID Microbiome Program, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD, USA., IRCCS Humanitas Research Hospital , Milan, Italy., Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Willem-Alexander Children's Hospital, Department of Pediatrics, Pediatric Stem Cell Transplantation Program, Leiden University Medical Center , Leiden, Netherlands., Neutrophil Monitoring Lab, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Pediatric Unit, Department of Clinical, Surgical, Diagnostic, and Pediatric Sciences, University of Pavia, Pavia, Italy., Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo , Pavia, Italy., Allergy and Immunology Division Hospital Nacional Edgardo Rebagliati Martins , Lima, Peru., Hospital Pediátrico, Unidade Local de Saúde de Coimbra , Coimbra, Portugal. Clínica Universitária de Pediatria, Faculdade de Medicina, Universidade de Coimbra , Coimbra, Portugal. Clinic for Special Children , Gordonville, PA, USA., Experimental Transplantation and Immunotherapy Branch, National Cancer Institute of the National Institutes of Health , Bethesda, MD, USA., Allergy and Immunology, Children's Healthcare of Atlanta, Emory University School of Medicine , Atlanta, GA, USA., Division of Immunology, Allergy and Rheumatology, Joe DiMaggio Children's Hospital, Memorial Healthcare System, Hollywood, FL, USA., Immuno-reumatologia, Pediatria Specialistica Universitaria, Ospedale Infantile Regina Margherita , Torino, Italy., Department of Pediatric Immunology, Mother and Child Health Institute, Medical Faculty, University of Belgrade, Belgrade, Serbia., Division of Pediatric Allergy/Immunology, University of South Florida at Johns Hopkins All Children's Hospital, St. Petersburg, FL, USA., Belarusian Research Center for Pediatric Oncology, Hematology and Immunology , Minsk, Belarus., Division of Allergy and Immunology, Children 39;s National Hospital, Washington, DC, USA., Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology , Brisbane, Australia., Cutaneous Microbiome and Inflammation Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA., Microbial Genomics Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA., San Raffaele-Telethon Institute for Gene Therapy (SR-Tiget), IRCSS San Raffaele Scientific Institute , Milan, Italy., Milan Unit, Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Milan, Italy., Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA., Department of Immunology, Institut Pasteur, Paris, France.,
    1. Year: 2025
    2. Date: Aug 04
    3. Epub Date: 2025 05 02
  2. Journal: The Journal of Experimental Medicine
    1. 222
    2. 8
  4. Type of Article: Article
  5. Article Number: e20241993
  1. Abstract:

    Partial RAG deficiency (pRD) can manifest with systemic and tissue-specific immune dysregulation, with inflammatory bowel disease (IBD) in 15% of the patients. We aimed at identifying the immunopathological and microbial signatures associated with IBD in patients with pRD and in a mouse model of pRD (Rag1w/w) with spontaneous development of colitis. pRD patients with IBD and Rag1w/w mice showed a systemic and colonic Th1/Th17 inflammatory signature. Restriction of fecal microbial diversity, abundance of pathogenic bacteria, and depletion of microbial species producing short-chain fatty acid were observed, which were associated with impaired induction of lamina propria peripheral Treg cells in Rag1w/w mice. The use of vedolizumab in Rag1w/w mice and of ustekinumab in a pRD patient were ineffective. Antibiotics ameliorated gut inflammation in Rag1w/w mice, but only bone marrow transplantation (BMT) rescued the immunopathological and microbial signatures. Our findings shed new light in the pathophysiology of gut inflammation in pRD and establish a curative role for BMT to resolve the disease phenotype. © 2025 Castagnoli et al.

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External Sources

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  2. DOI: 10.1084/jem.20241993
  3. PMID: 40314722
  4. PMCID: PMC12047384
  5. PII : 277670

Library Notes

  1. Fiscal Year: FY2024-2025
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