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Genetic Redundancy Between Spt23 and Mga2 - Regulators of Ty-Induced Mutations and Ty1 Transcription in Saccharomyces Cerevisiae

  1. Author:
    Zhang, S. R.
    Burkett, T. J.
    Yamashita, I.
    Garfinkel, D. J.

  2. Author Address

    Garfinkel DJ NCI GENE REGULAT & CHROMOSOME BIOL LAB FREDERICK CANC RES & DEV CTR ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA NCI GENE REGULAT & CHROMOSOME BIOL LAB FREDERICK CANC RES & DEV CTR ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA HIROSHIMA UNIV CTR GENE SCI HIGASHIHIROSHIMA 724 JAPAN
    1. Year: 1997
  2. Journal: Molecular and Cellular Biology
    1. 17
    2. 8
    3. Pages: 4718-4729
  4. Type of Article: Article
  1. Abstract:

    SPT23 was isolated as a dosage-dependent suppressor of Ty-induced mutations in Saccharomyces cerevisiae. SPT23 shows considerable sequence homology with MGA2, a gene identified as a dosage-dependent suppressor of a snf2-imposed block on STA1 transcription in S. cerevisiae var. diastaticus. Although single mutations in either of these genes have only modest effects on cell growth, spt23 mga2 double mutants are inviable. Unlike SPT23, multicopy expression of a truncated form of MGA2 suppresses a narrow subset of Ty-induced mutations. SPT23/MGA2 and the SNF/SWI genes affect transcription of certain target genes in similar ways, Spt23p appears to be a rate-limiting component required for functional HIS4 expression of his4-912 delta, a promoter insertion mutation induced by the Tyl-912 long terminal repeat, Furthermore, both Spt23p and Mga2p can activate transcription when fused to the Gal4p DNA-binding domain, as previously observed with Snf2p and Snf5p. A 50-amino-acid region in the N terminus of the predicted Spt23p protein is necessary and sufficient for the transactivation and necessary for suppression of Ty1-induced mutations and the essential function of Spt23p. Cell fractionation and cytological experiments suggest that Spt23p is associated with the nucleus. Our results suggest that SPT23/MGA2 affects transcription of a subset of genes in yeast, perhaps by changing chromatin accessibility. [References: 65]

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