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Generation of macrophages from early T progenitors in vitro

  1. Author:
    Lee, C. K.
    Kim, J. K.
    Kim, Y.
    Lee, M. K.
    Kim, K.
    Kang, J. K.
    Hofmeister, R.
    Durum, S. K.
    Han, S. S.

  2. Author Address

    Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea. Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea. Chungbuk Natl Univ, Coll Vet Med, Cheongju 361763, South Korea. Sahm Yook Univ, Dept Pharm, Seoul, South Korea. NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. Lee CK Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea.
    1. Year: 2001
  2. Journal: Journal of Immunology
    1. 166
    2. 10
    3. Pages: 5964-5969
  4. Type of Article: Article
  1. Abstract:

    Early T progenitors in the thymus have been reported to have the capacity to develop into B cells, thymic dendritic cells, and NK cells. Here we describe conditions that induce early T progenitors to develop into macrophages. Initially, we observed that early T progenitors could be induced to develop into macrophages by cytokines produced from a thymic stromal cell line, TFGD, and later we found that the cytokine mixture of M- CSF plus IL-6 plus IL-7 also induced macrophage differentiation from pro-T cells. M-CSF by itself was unable to induce macrophage differentiation from early T progenitors. To correlate this observation with the developmental potential of early T progenitors, mouse embryonic thymocytes were sorted into four populations, pro-T1 to pro-T4, based on the expression of CD44 and CD25, and then cultured with TFGD culture supernatant. We found that pro-T1 and pro-T2 cells, but not pro-T3 and pro-T4 cells, generate macrophages. Limiting dilution analysis of the differentiation capability of sorted pro-T2 cells also confirmed that pro-T2 cells could generate macrophages. These results suggest that T cells and thymic macrophages could originate from a common intrathymic precursor.

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