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Equine infectious anemia virus and the ubiquitin-proteasome system

  1. Author:
    Ott, D. E.
    Coren, L. V.
    Sowder, R. C.
    Adams, J.
    Nagashima, K.
    Schubert, U.

  2. Author Address

    Natl Canc Inst Frederick, SAIC Frederick, AIDS Vaccine Program, Ft Detrick, MD 21702 USA. Natl Canc Inst Frederick, SAIC Frederick, AIDS Vaccine Program, Ft Detrick, MD 21702 USA. Natl Canc Inst Frederick, SAIC Frederick, Res Technol Program, Ft Detrick, MD 21702 USA. Millennium Pharmaceut, Cambridge, MA 02139 USA. NIAID, Lab Viral Dis, NIH, Bethesda, MD 20892 USA. Heinrich Pette Inst, D-20251 Hamburg, Germany. Ott DE Natl Canc Inst Frederick, SAIC Frederick, AIDS Vaccine Program, Ft Detrick, MD 21702 USA.
    1. Year: 2002
  2. Journal: Journal of Virology
    1. 76
    2. 6
    3. Pages: 3038-3044
  4. Type of Article: Article
  1. Abstract:

    Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and retrovirus assembly. We examined equine infectious anemia virus (EIAV) particles and found that approximately 2% of the p9(Gag) proteins are monoubiquitinated, demonstrating that this Gag protein interacts with an ubiquitinating activity. Different types of proteasome inhibitors were used to determine if proteasome Inactivation affects EIAV release from chronically infected cells. Pulse-chase immunoprecipitation and time course immunoblot analyses showed that proteasome inactivation slightly decreased virus release (at most a twofold effect), while it did not affect Gag processing. These results contrast with those obtained with other viruses which are sensitive to these inhibitors. This suggests that, although its Gag is monoubiquitinated, the requirements for EIAV release are somewhat different from those for retroviruses that are sensitive to proteasome inhibitors.

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