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Effect of Mycobacterium bovis BCG vaccination on mycobacterium- specific cellular proliferation and tumor necrosis factor alpha production from distinct guinea pig leukocyte populations

  1. Author:
    Lasco, T. M.
    Yamamoto, T.
    Yoshimura, T.
    Allen, S. S.
    Cassone, L.
    McMurray, D. N.

  2. Author Address

    Colorado State Univ, Dept Microbiol Immunol & Pathol, 320B Microbiol Bldg,1682 Campus Delivery, Ft Collins, CO 80523 USA Texas A&M Univ Syst, Hlth Sci Ctr, Dept Med Microbiol & Immunol, College Stn, TX 77843 USA Texas A&M Univ, Coll Vet Med, College Stn, TX 77843 USA NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA Lasco TM Colorado State Univ, Dept Microbiol Immunol & Pathol, 320B Microbiol Bldg,1682 Campus Delivery, Ft Collins, CO 80523 USA
    1. Year: 2003
  2. Journal: Infection and Immunity
    1. 71
    2. 12
    3. Pages: 7035-7042
  4. Type of Article: Article
  1. Abstract:

    In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-alpha) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-alpha from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.

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