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Exploring the relationship between serotonin and brain-derived neurotrophic factor: analysis of BDNF protein and extraneuronal 5-HT in mice with reduced serotonin transporter or BDNF expression

  1. Author:
    Szapacs, M. E.
    Mathews, T. A.
    Tessarollo, L.
    Lyons, W. E.
    Mamounas, L. A.
    Andrews, A. M.
  2. Author Address

    Penn State Univ, Dept Chem, University Pk, PA 16802 USA. Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. NCI, Neurol Dev Grp, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NCI, Mol Embryol Sect, Frederick Canc Res & Dev Ctr, ABL,Basic Res Program, Frederick, MD 21702 USA. NINDS, NIH, Sci Review Branch, Ctr Neurosci, Bethesda, MD 20892 USA. NINDS, NIH, Extramural Res Program, Ctr Neurosci, Bethesda, MD 20892 USA Andrews, AM, Penn State Univ, Dept Chem, 152 Davey Lab, University Pk, PA 16802 USA
    1. Year: 2004
    2. Date: DEC 30
  1. Journal: Journal of Neuroscience Methods
    1. 140
    2. 1-2
    3. Pages: 81-92
  2. Type of Article: Article
  1. Abstract:

    Serotonin (5-HT) has been proposed to promote neuronal plasticity during the treatment of mood and anxiety disorders and following neurodegenerative insult by altering the expression of critical genes including brain-derived neurotrophic factor (BDNF). In this study mice with constitutive reductions in the serotonin transporter (SERT) or BDNF were investigated to further assess the functional relationship between serotonin neurotransmission and BDNF expression. Using a modified extraction procedure and a commercial enzyme-linked immunosorbant assay, 50% decreases in BDNF protein in hippocampus, frontal cortex and brain stem were confirmed in 4-month-old mice lacking one copy, of the BDNF gene (BDNF+/-). By contrast, 4-month-old male and female mice with partial (SERT+/-) or complete (SERT-/-) reductions in SERT expression showed no differences in BDNF protein levels compared to SERT+/+ mice. although male SERT knockout mice of all genotypes had higher BDNF levels in hippocampus, frontal cortex. and brain stern than female animals. Microdialysis also was performed in BDNF+/- mice. In addition to other phenotypic aspects suggestive of altered serotonin neurotransmission. BDNF+/- mice show, accelerated age-related degeneration of 5-HT forebrain innervation. Nevertheless. extracellular 5-HT levels determined by zero net flux microdialysis were similar between BDNF+/+ and BDNF+/- mice in striatum and frontal cortex at 8-12 months of age. These data illustrate that a 50%. decrease in BDNF does not appear to be sufficient to cause measurable changes in basal extracellular 5-HT concentrations and, furthermore. that constitutive reductions in SERT expression are not associated with altered BDNF protein levels at the ages and in the brain re-pons examined in this study. (C) 2004 Elsevier B.V. All rights reserved

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External Sources

  1. DOI: 10.1016/j.jneumeth.2004.03.026
  2. WOS: 000226180100013

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