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Proteomic investigation of natural killer cell microsomes using gas-phase fractionation by mass spectrometry

  1. Author:
    Blonder, J.
    Rodriguez-Galan, M. C.
    Lucas, D. A.
    Young, H. A.
    Issaq, H. J.
    Veenstra, T. D.
    Conrads, T. P.

  2. Author Address

    Conrads, TP, NCI, SAIC Frederick Inc, Lab Proteom & Analyt Technol, Biomed Proteom Program, POB B, Frederick, MD 21702 USA NCI, SAIC Frederick Inc, Lab Proteom & Analyt Technol, Biomed Proteom Program, Frederick, MD 21702 USA. NCI, Expt Immunol Lab, Frederick, MD 21702 USA.
    1. Year: 2004
  2. Journal: Biochimica Et Biophysica Acta-Proteins and Proteomics
    1. 1698
    2. 1
    3. Pages: 87-95
  4. Type of Article: Article
  1. Abstract:

    We have explored the utility of gas-phase fractionation by mass spectrometry (MS) in the mass-to-charge (m/z) dimension (GPF(m/z)) for increasing the effective number of protein identifications in cases where sample quantity limits the use of multi-dimensional chromatographic fractionation. A peptide digestate from proteins isolated from the membrane fraction of natural killer (NK) cells was analyzed by microcapillary reversed-phase liquid chromatography coupled online to an ion-trap (IT) mass spectrometer. Performing GPF(m/z). using eight narrow precursor ion scan m1z ranges enabled the identification of 340 NK cell proteins from 12 mug of digestate, representing more than a fivefold increase in the number of proteins identified as compared to the same experiment employing a standard precursor ion survey scan m/z range (i.e., m/z 400-2000). The results show that GPF(m/z) represents an effective technique for increasing protein identifications in global proteomic investigations especially when sample quantity is limited. (C) 2003 Elsevier B.V. All rights reserved

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  2. DOI: 10.1016/j.bbapap.2003.10.009
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