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The effect of flavonoids on DMBA-DNA adduct formation in MCF-7 human breast cancer cells

  1. Author:
    Ciolino, H. P.
    Yeh, G. C.
    1. Year of Conference: 1997
  2. Conference Name: Annual Meeting of the American Association for Cancer Research
    1. 38
    2. Pages: A3890
  4. Type of Work: Meeting Abstract
  1. Abstract:

    7,12-dimethylbenzanthracene (DMBA) is a mammary carcinogen that is metabolized in cells by cytochrome P450, forming activated compounds which bind specific residues of DNA and initiate carcinogenesis. In the present study we have used the MCF-7 human breast cancer cell line to examine the mechanism of DMBA-DNA adduct formation and screen possible dietary effectors of this process. Adduct formation in MCF-7 cells exposed to [3H]-DMBA was dose- and time-dependent. Flavonoids, dietary compounds found in plants and known to have chemopreventive effects, reduced or eliminated adduct formation in cells in a dose-dependent fashion. Cytochrome P4501A (CYP1A) activity was not measurable in untreated cells but was induced in cells exposed to DMBA or other polycyclic aromatic hydrocarbons such as benzo(a)pyrene. The flavonoids which prevented adduct formation also prevented the induction of CYP1A. These results indicate that dietary factors such as the flavonoids may affect the carcinogenic potential of polycyclic aromatic hydrocarbons by causing alterations in their metabolism.

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