Skip NavigationSkip to Content
Return Return to Search Results

Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae

  1. Author:
    Ulrich, R. L.
    Ulrich, M. P.
    Schell, M. A.
    Kim, H. S.
    DeShazer, D.

  2. Author Address

    USA, Med Res Inst Infect Dis, Bacteriol Div, Ft Detrick, Frederick, MD 21702 USA. USA, Med Res Inst Infect Dis, Diagnost Syst Div, Ft Detrick, Frederick, MD 21702 USA. Univ Georgia, Dept Microbiol, Athens, GA 30602 USA. Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. Inst Genom Res, Rockville, MD 20850 USA.;DeShazer, D, USA, Med Res Inst Infect Dis, Bacteriol Div, Ft Detrick, Frederick, MD 21702 USA.;david.deshazer@amedd.army.mil
    1. Year: 2006
    2. Date: May
  2. Journal: Diagnostic Microbiology and Infectious Disease
    1. 55
    2. 1
    3. Pages: 37-45
  4. Type of Article: Article
  5. ISSN: 0732-8893
  1. Abstract:

    Burkholderia mallei and Burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category B biothreat agents. We used an in silico approach to compare the B. mallei ATCC 23344 and B. pseudomallei K96243 genomes to identify nucleotide sequences unique to B. mallei. Five distinct B. mallei DNA sequences and/or genes were identified and evaluated for polymerase chain reaction (PCR) assay development. Genomic DNAs from a collection of 31 B. mallei and 34 B. pseudomallei isolates, obtained from various geographic, clinical, and environmental sources over a 70-year period, were tested with PCR primers targeted for each of the B. mallei ATCC 23344-specific nucleotide sequences. Of the 5 chromosomal targets analyzed, only PCR primers designed to bimA(Bm) were specific for B. mallei. These primers were used to develop a rapid PCR assay for the definitive identification of B. mallei and differentiation from all other bacteria. (c) 2006 Elsevier Inc. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.diagmicrobio.2005.11.007
  3. View record in Web of Science®
  4. WOS: 000237910800006

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel