Skip NavigationSkip to Content
Return Return to Search Results

Coassembly and complementation of Gag proteins from HIV-1 and HIV-2, two distinct human pathogens

  1. Author:
    Boyko, V.
    Leavitt, M.
    Gorelick, R.
    Fu, W.
    Nikolaitchik, O.
    Pathak, V. K.
    Nagashima, K.
    Hu, W. S.

  2. Author Address

    SAIC Frederick Inc, HIV Drug Resistance Program, NCI, Frederick, MD 21702 USA. SAIC Frederick Inc, AIDS Vaccine Program, Basic Res Program, NCI, Frederick, MD 21702 USA. SAIC Frederick Inc, Image Anal Lab, NCI, Frederick, MD 21702 USA.;Hu, WS, SAIC Frederick Inc, HIV Drug Resistance Program, NCI, Frederick, MD 21702 USA.;whu@ncifcrf.gov
    1. Year: 2006
    2. Date: Jul
  2. Journal: Molecular Cell
    1. 23
    2. 2
    3. Pages: 281-287
  4. Type of Article: Article
  5. ISSN: 1097-2765
  1. Abstract:

    Approximately one million people in the world are dually infected with both HIV-1 and HIV-2. To identity potential interactions between these two human pathogens, we examined whether HIV-1 and HIV-2 Gag proteins can coassemble and functionally complement each other. We generated HIV-1 - and HIV-2-based vectors with mutations in Gag; compared with wild-type vectors, these mutants had drastically decreased viral titers. Coexpression of the mutant HIV-1 and HIV-2 Gag could generate infectious viruses; furthermore, heterologous complementation in certain combinations showed efficiency similar to homologous complementation. Additionally, we used bimolecularfluorescence complementation analysis to directly demonstrate that HIV-1 and HIV-2 Gag can interact and coassemble. Taken together, our results indicate that HIV-1 and HIV-2 Gag polyproteins can coassemble and functionally complement each other during virus replication; to our knowledge, this is the first demonstration of its kind. These studies have important implications for AIDS treatment and the evolution of primate lentiviruses.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.molcel.2006.05.028
  3. View record in Web of Science®
  4. WOS: 000239647700016

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel