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Conformationally constrained analogues of diacylglycerol (DAG). 28. DAG-dioxolanones reveal a new additional interaction site in the C1b domain of PKC delta

  1. Author:
    Choi, Y.
    Pu, Y.
    Peach, M. L.
    Kang, J. H.
    Lewin, N. E.
    Sigano, D. M.
    Garfield, S. H.
    Blumberg, P. M.
    Marquez, V. E.

  2. Author Address

    NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NCI, Expt Carcinogenesis Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NCI, Med Chem Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA. SAIC Frederick Inc, NCI Frederick, Basic Res Program, Frederick, MD 21702 USA.;Blumberg, PM, NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.;ychoi@korea.ac.kr blumberp@dc37a.nci.nih.gov marquezv@mail.nih.gov
    1. Year: 2007
    2. Date: Jul
  2. Journal: Journal of Medicinal Chemistry
    1. 50
    2. 15
    3. Pages: 3465-3481
  4. Type of Article: Article
  5. ISSN: 0022-2623
  1. Abstract:

    Diacylglycerol (DAG) lactones have provided a powerful platform for structural exploration of the interactions between ligands and the C1 domains of protein kinase C (PKC). In this study, we report that DAG-dioxolanones, novel derivatives of DAG-lactones, exploit an additional point of contact (glutamine 27) in their binding with the C1b domain of PKC delta. Mutation of this point of contact to glutamate selectively impairs binding of the DAG-dioxolanones compared to that of the corresponding DAG-lactones (1200- to 3000-fold versus 35- to 55-fold, respectively). The differential response of this mutated C1b domain to the DAG-dioxolanones relative to the DAG-lactones provides a unique tool to probe the role of the C1b domain in PKC delta function, where the response to the DAG-lactones affords a positive control for retained function. Using this approach, we show that the C1b domain of PKC delta plays the predominant role in the translocation of PKC delta to the membrane in the presence of DAG.

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  2. DOI: 10.1021/jm0702579
  3. View record in Web of Science®
  4. WOS: 000248121300011

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