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Nitric oxide is a key component in inflammation-accelerated tumorigenesis

  1. Author:
    Hussain, S. P.
    He, P.
    Subleski, J.
    Hofseth, L. J.
    Trivers, G. E.
    Mechanic, L.
    Hofseth, A. B.
    Bernard, M.
    Schwank, J.
    Nguyen, G.
    Mathe, E.
    Djurickovic, D.
    Haines, D.
    Weiss, J.
    Back, T.
    Gruys, E.
    Laubach, V. E.
    Wiltrout, R. H.
  2. Author Address

    Hussain, S. Perwez, He, Peijun] NCI, Human Carcinogenesis Lab, NIH, Ctr Canc Res, Bethesda, MD 20892 USA. [Nguyen, Giang] NIH, Howard Hughes Med Inst, Bethesda, MD 20892 USA. [Subleski, Jeffery, Weiss, Jonathan, Back, Timothy, Gruys, Eilene, Wiltrout, Robert H.] NCI, Expt Immunol Lab, Canc Inflammat Program, Ctr Canc Res, Frederick, MD 21701 USA. [Haines, Diana] Frederick Canc Res & Dev Ctr, Sci Applicat Int Corp, Frederick, MD USA. [Hofseth, Lorne J.; Hofseth, Anne B.] Univ S Carolina, Dept Basic Pharm Sci, Coll Pharm, Columbia, SC 29208 USA. [Laubach, Victor E.] Univ Virginia, Hlth Sci Ctr, Charlottesville, VA USA. [Mechanic, Leah] WESTAT Corp, Rockville, MD 20850 USA.
    1. Year: 2008
  1. Journal: Cancer Research
    1. 68
    2. 17
    3. Pages: 7130-7136
  2. Type of Article: Article
  1. Abstract:

    Nitric oxide (NO center dot), an important signaling molecule and it component of inflammatory response, is involved in tumorigenesis. However, the quantity of NO center dot and the cellular micro-environment influences the role of NO center dot in tumor development. We used a genetic strategy to test the hypothesis that an inflammatory microenvironment with an enhanced level of NO center dot accelerates spontaneous tumor development. C. parvum-induced inflammation and increased NO center dot synthase-2 (NOS2) expression coincided with accelerated spontaneous tumor development, mostly lymphomas, in p53-/-NOS2+/+ C57BL6 mice when compared with the controls (P = 0.001). However, p53-/-NOS2-/- mice did not show any difference in tumor latency between C. parvum-treated and control groups. In C. parvum-treated p53-/-NOS2+/+ mice, tumor development was preceded by a higher expression of NOS2 and phosphorylated Akt-Ser(473) (pAkt-Ser473) in spleen, increased cell proliferation measured by Ki-67 IHC in spleen and thymus, and a lower apoptotic index and CD95-L expression in spleen and thymus. C. parvum-treated p53-/-NOS2+/+ mice showed an increase in the number of Foxp3(+) T-reg cells, dendritic cells (I)Q, as well as increased CD80(+), CD86(+), CD40(+), and CD83(+) on DC in the spleen. Regulatory T-cells (T-reg) and the maturation of DC may modulate tumorigenesis. An increase in the FoxP3(+)T-reg cells in C. parvum-treated p53-/-NOS2+/+ mice indicates a role of NO center dot in the regulation of T-reg cells that may contribute to a protumor shift of the immune environment favoring an accelerated tumor development. These data provide genetic and mechanistic evidence that an inflammatory microenvironment and an increased level of NO center dot can accelerate tumor development.

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External Sources

  1. PMID: 18757428

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