Skip NavigationSkip to Content
Return Return to Search Results

Non-homologous end joining is important for repair of Cr(VI)-induced DNA damage in Saccharomyces cerevisiae

  1. Author:
    Santoyo, G.
    Strathern, J. N.

  2. Author Address

    Santoyo, Gustavo, Strathern, Jeffrey N.] NCI, Gene Regulat & Chromosome Biol Lab, NIH, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Microbiological Research
    1. 163
    2. 1
    3. Pages: 113-119
  4. Type of Article: Article
  1. Abstract:

    Hexavalent chromium is known to be a potent carcinogen that leads to many different DNA lesions, including DNA - protein crosslinks, and single- and doubte-strand breaks. In Saccharomyces cerevisiae, DNA double-strand breaks are mainly repaired by either homologous recombination (HR) or non-homologous end-joining (NHEJ) repair pathways. Here, we show that mutants deficient in NHEJ (yku70 Delta, rad50 Delta, dnl4 Delta, mre11 Delta, xrs2 Delta) of S. cerevisiae are more sensitive to Cr(VI) toxic effects than wild-type cells. Also, a deletion mutant of SAE2 showed a similar sensitivity to Cr(VI), even though it has no apparent direct role in NHEJ. We also found that double mutants in HR and NHEJ (yku70 Delta/rad52 Delta, rad50 Delta/rad52 Delta, dnl4 Delta/rad52 Delta, mre11 Delta/rad52 Delta, xrs2 Delta/rad52 Delta) are synergistically more sensitive to Cr(VI) exposure than any of the single mutants, indicating that both repair pathways are involved in the repair of Cr(Vl)-induced lesions. Finally, when the NHEJ mutants were exposed to Cr(VI) under anaerobic growth conditions, Cr(VI) toxicity was suppressed. (c) 2007 Elsevier GmbH. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. PMID: 17923397

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel