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Antifungal activity of recombinant mouse beta-defensin 3

  1. Author:
    Jiang, Y.
    Wang, Y.
    Wang, B.
    Yang, D.
    Yu, K.
    Yang, X.
    Liu, F.
    Jiang, Z.
    Li, M.

  2. Author Address

    [Jiang, Y.; Wang, Y.; Wang, B.; Yu, K.; Yang, X.; Liu, F.; Jiang, Z.; Li, M.] Sichuan Univ, Dept Microbiol, W China Sch Preclin & Forens Med, Chengdu 610041, Peoples R China. [Jiang, Y.] Guiyang Med Coll, Dept Microbiol, Guiyang 550004, Peoples R China. [Yang, D.] SAIC Frederick, Basic Res Program, Frederick, MD 21702 USA. [Yang, D.] NCI, Mol Immunoregulat Lab, NCI FCRDC, NIH, Frederick, MD 21702 USA. [Li, M.] Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China.;Li, M, Sichuan Univ, Dept Microbiol, W China Sch Preclin & Forens Med, Chengdu 610041, Peoples R China.;lmy3985@sina.com
    1. Year: 2010
    2. Date: May
  2. Journal: Letters in Applied Microbiology
    1. 50
    2. 5
    3. Pages: 468-473
  4. Type of Article: Article
  5. ISSN: 0266-8254
  1. Abstract:

    Aims: To identify the presence of mouse beta-defensin 3 (Mbd3) (the human homologue of beta-defensin 2) in different tissues and to define the antimicrobial properties of recombinant MBD3 (rMBD3) against a panel of human pathogens. Methods and Results: Mbd3 gene expression in different mouse tissues before or after lipopolysaccharide (LPS) injection was compared by semi-quantitative RT-PCR. This analysis demonstrated that epithelial and mucosal tissues expressed Mbd3 independent of LPS stimulation. Evaluation of the antimicrobial properties of recombinant rMBD3 was determined by assessing the median inhibition concentration (IC50), minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)/minimal fungicidal concentration (MFC) against various human pathogens. Conclusion: Mbd3 gene expression by epithelial and mucosal tissues suggested that MBD3 likely plays an early defensive role against microbial infections. This activity was most significant against filamentous fungi. Significance and Impact of the Study: The data presented in this report suggested that formulations containing rMBD3 and related molecules could serve to treat fungal and bacterial infections.

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External Sources

  1. View Full Text
  2. DOI: 10.1111/j.1472-765X.2010.02824.x
  3. View record in Web of Science®
  4. WOS: 000276406400005

Library Notes

  1. Fiscal Year: FY2009-2010
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