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Kaposi Sarcoma (KS)-Associated Herpesvirus MicroRNA Sequence Analysis and KS Risk in a European AIDS-KS Case Control Study

  1. Author:
    Marshall, V.
    Martro, E.
    Labo, N.
    Ray, A.
    Wang, D. A.
    Mbisa, G.
    Bagni, R. K.
    Volfovsky, N.
    Casabona, J.
    Whitby, D.
    Grp, E.-S. S.

  2. Author Address

    [Marshall, Vickie; Labo, Nazzarena; Ray, Alex; Wang, Dian; Mbisa, Georginia; Whitby, Denise] NCI, Viral Oncol Sect, AIDS & Canc Virus Program, SAIC Frederick, Frederick, MD 21702 USA. [Bagni, Rachel K.] NCI, Mol Detect Grp, Prot Express Lab, Adv Technol Program,SAIC Frederick, Frederick, MD 21702 USA. [Volfovsky, Natalia] NCI, Adv Biomed Comp Ctr, Informat Syst Program, SAIC Frederick, Frederick, MD 21702 USA. [Martro, Elisa] Hosp Badalona Germans Trias & Pujol, Microbiol Serv, Badalona, Spain. [Casabona, Jordi] Hosp Badalona Germans Trias & Pujol, Dept Salut, Ctr Estudis Epidemiol HIV SIDS Catalunya, Badalona, Spain. [Martro, Elisa] CIBER Epidemiol & Salud Publ, Madrid, Spain.;Whitby, D, NCI, Viral Oncol Sect, AIDS & Canc Virus Program, SAIC Frederick, POB B, Frederick, MD 21702 USA.;whitbyd@mail.nih.gov
    1. Year: 2010
    2. Date: Oct 1
    3. Epub Date: 8/19/2010
  2. Journal: Journal of Infectious Diseases
    1. 202
    2. 7
    3. Pages: 1126-1135
  4. Type of Article: Article
  5. ISSN: 0022-1899
  1. Abstract:

    Background. We recently identified polymorphisms in Kaposi sarcoma-associated herpesvirus (KSHV)-encoded microRNA (miRNA) sequences from clinical subjects. Here, we examine whether any of these may contribute to KS risk in a European AIDS-KS case-control study. Methods. KSHV load in peripheral blood was determined by real-time quantitative polymerase chain reaction. Samples that had detectable viral loads were used to amplify the 2.8-kb miRNA encoding region plus a 646-bp fragment of the K12/T0.7 gene. Additionally, we characterized an 840-bp fragment of the K1 gene to determine KSHV subtypes. Results. KSHV DNA was detected in peripheral blood mononuclear cells of 49.6% of case patients and 6.8% of controls, and viral loads tended to be higher in case patients. Sequences from the miRNA-encoding regions were conserved overall, but distinct polymorphisms were detected, some of which occurred in primary miRNAs, pre-miRNAs, or mature miRNAs. Conclusions. Patients with KS were more likely to have detectable viral loads than were controls without disease. Despite high conservation in KSHV miRNA-encoded sequences, polymorphisms were observed, including some that have been reported elsewhere. Some polymorphisms could affect mature miRNA processing and appear to be associated with KS risk.

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External Sources

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  2. DOI: 10.1086/656045
  3. PMID: 20715927
  4. PMCID: PMC2932837
  5. View record in Web of Science®
  6. WOS: 000281912100017
  7. NIHMSID: Nihms218288

Library Notes

  1. Fiscal Year: FY2010-2011
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