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Trafficking of a Dual-Modality Magnetic Resonance and Fluorescence Imaging Superparamagnetic Iron Oxide-Based Nanoprobe to Lymph Nodes

  1. Author:
    Bumb, A.
    Regino, C. A. S.
    Egen, J. G.
    Bernardo, M.
    Dobson, P. J.
    Germain, R. N.
    Choyke, P. L.
    Brechbiel, M. W.

  2. Author Address

    [Bumb, A; Brechbiel, MW] NCI, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA. [Regino, CAS; Bernardo, M; Choyke, PL] NCI, Mol Imaging Program, NIH, Bethesda, MD 20892 USA. [Egen, JG; Germain, RN] NIAID, Lab Immunol, NIH, Bethesda, MD 20892 USA. [Bernardo, M] NCI, SAIC Frederick Inc, Frederick, MD 21702 USA. [Choyke, PL] Univ Oxford, Oxford OX5 1PF, England. [Brechbiel, MW] NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA.;Brechbiel, MW (reprint author), NCI, Radiat Oncol Branch, NIH, Bldg 10, Bethesda, MD 20892 USA;martinwb@mail.nih.gov
    1. Year: 2011
    2. Date: Dec
  2. Journal: Molecular Imaging and Biology
    1. 13
    2. 6
    3. Pages: 1163-1172
  4. Type of Article: Article
  5. ISSN: 1536-1632
  1. Abstract:

    Purpose: This study aims to develop and characterize the trafficking of a dual-modal agent that identifies primary draining or sentinel lymph node (LN). Procedure: Herein, a dual-reporting silica-coated iron oxide nanoparticle (SCION) is developed. Nude mice were imaged by magnetic resonance (MR) and optical imaging and axillary LNs were harvested for histological analysis. Trafficking through lymphatics was observed with intravital and ex vivo confocal microscopy of popliteal LNs in B6-albino, CD11c-EYFP, and lys-EGFP transgenic mice. Results: In vivo, SCION allows visualization of LNs. The particle's size and surface functionality play a role in its passive migration from the intradermal injection site and its minimal uptake by CD11c+ dendritic cells and CD169+ and lys+ macrophages. Conclusions: After injection, SCION passively migrates to LNs without macrophage uptake and then can be used to image LN(s) by MRI and fluorescence. Thus, SCION can potentially be developed for use in sentinel node resections or for intralymphatic drug delivery.

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External Sources

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  2. DOI: 10.1007/s11307-010-0424-8
  3. View record in Web of Science®
  4. WOS: 000296794400013

Library Notes

  1. Fiscal Year: FY2011-2012
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