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Structure of the yeast U2/U6 snRNA complex

  1. Author:
    Burke, J. E.
    Sashital, D. G.
    Zuo, X. B.
    Wang, Y. X.
    Butcher, S. E.

  2. Author Address

    [Burke, Jordan E.; Sashital, Dipali G.; Butcher, Samuel E.] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA. [Zuo, Xiaobing] Argonne Natl Lab, Adv Photon Source, Chicago, IL 60437 USA. [Wang, Yun-Xing] NCI, NIH, Frederick, MD 21702 USA.;Butcher, SE (reprint author), Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA;butcher@biochem.wisc.edu
    1. Year: 2012
    2. Date: Apr
  2. Journal: Rna-a Publication of the Rna Society
    1. 18
    2. 4
    3. Pages: 673-683
  4. Type of Article: Article
  5. ISSN: 1355-8382
  1. Abstract:

    The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended "Y'' shape. The U6 internal stem-loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex-including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites-localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis.

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External Sources

  1. View Full Text
  2. DOI: 10.1261/rna.031138.111
  3. View record in Web of Science®
  4. WOS: 000301954600007

Library Notes

  1. Fiscal Year: FY2011-2012
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