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The Examination of Viral Characteristics of HIV-1 CRF07_BC and Its Potential Interaction with Extracellular Galectin-3

  1. Author:
    Lin, Chih-Yen
    Wang, Wen-Hung
    Huang,Szu-Wei
    Yeh, Chun-Sheng
    Yuan, Ruei-Yu
    Yang, Zih-Syuan
    Urbina, Aspiro Nayim
    Tseng, Sung-Pin
    Lu, Po-Liang
    Chen, Yen-Hsu
    Wang, Seng-Fan [ORCID]

  2. Author Address

    Center for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung 80708, Taiwan., Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan., Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical, University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan., Model Development Section, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 20892, USA., Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.,
    1. Year: 2020
    2. Date: JUN
    3. Epub Date: 2020 05 29
  2. Journal: Pathogens (Basel, Switzerland)
    1. 9
    2. 6
    3. Pages: E425
  4. Type of Article: Article
  5. Article Number: 425
  6. ISSN: 2076-0817
  1. Abstract:

    HIV-1 CRF07_BC is a B 39; and C subtype recombinant emerging virus and many of its viral characteristics remain unclear. Galectin-3 (Gal3) is a ß-galactose binding lectin that has been reported as a pattern recognition receptor (PRR) and is known to mediate adhesion between cells and microbes. This study aims to examine the viral characteristics of HIV-1 CRF07_BC virus and the role of extracellular galectin-3 in HIV-1 CRF07_BC infection. A total of 28 HIV-1+ injecting drug users (IDUs) were recruited and 24 (85.7%) were identified as HIV-1 CRF07_BC. Results indicate that significant higher serum galectin-3 was measured in CRF07_BC infected patients and CRF07_BC infection triggered significant galectin-3 expression (p < 0.01). Viral characteristics demonstrate that CRF07_BC virions display a higher level of envelope gp120 spikes. The virus infectivity assay demonstrated that co-treatment with galectin-3 significantly promoted CRF07_BC attachment and internalization (p < 0.01). A co-immunoprecipitation assay showed that pulldown galectin-3 co-precipitated both CD4 and gp120 proteins. Results from an enzyme-linked immunosorbent assay (ELISA) indicate that the galectin-3 promoting effect occurs through enhancement of the interaction between gp120 and CD4. This study suggests that CRF07_BC was predominant in HIV-1+ IDUs and CRF07_BC utilized extracellular galectin-3 to enhance its infectivity via stabilization of the gp120-CD4 interaction.

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External Sources

  1. View Full Text
  2. DOI: 10.3390/pathogens9060425
  3. PMID: 32485969
  4. View record in Web of Science®
  5. WOS: 000551562300001
  6. PII : pathogens9060425

Library Notes

  1. Fiscal Year: FY2019-2020
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