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In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the raindance ddPCR platform

  1. Author:
    Long, Samuel

  2. Author Address

    AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, United States. Electronic address: Samuel.Long@nih.gov.,
    1. Year: 2021
    2. Date: Apr 9
  2. Journal: Methods
    1. 201
    2. Pages: 82-95
  4. Type of Article: Article
  1. Abstract:

    Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports indicate that digital PCR-based tests are significantly more sensitive than traditional qPCR tests. Numerous factors can influence digital PCR reaction sensitivity. In this review, using a model for human HIV infection and the Raindance ddPCR platform as an example, we describe technical aspects that contribute to sensitive viral signal detection in DNA and RNA from tissue samples, which often harbor viral reservoirs and serve as better predictors of disease outcome and indicators of treatment efficacy. Copyright © 2021. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.ymeth.2021.04.008
  3. PMID: 33839286
  4. PMCID: PMC8501152
  5. View record in Web of Science®
  6. WOS: 000810774500010
  7. PII : S1046-2023(21)00100-6

Library Notes

  1. Fiscal Year: FY2020-2021
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