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An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics

  1. Author:
    Borhani, Shayan G
    Levine, Max Z
    Krumpe,Lauren
    Wilson,Jennifer
    Henrich,Curtis
    O'Keefe,Barry
    Lo, Donald C
    Sittampalam, G Sitta
    Godfrey, Alexander G
    Lunsford, R Dwayne
    Mangalampalli, Venkata
    Tao, Dingyin
    LeClair, Christopher A
    Thole, Aaron P
    Frey, Douglas
    Swartz, James
    Rao, Govind

  2. Author Address

    Center for Advanced Sensor Technology, Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA., Department of Chemical Engineering and Department of Bioengineering, Stanford University, Stanford, CA 94305-5025, USA., Molecular Targets Program, Center for Cancer Research, NCI-Frederick, NIH, Frederick, MD 21702, USA; Basic Science Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA., Molecular Targets Program, Center for Cancer Research, NCI-Frederick, NIH, Frederick, MD 21702, USA., Molecular Targets Program, Center for Cancer Research, NCI-Frederick, NIH, Frederick, MD 21702, USA; Natural Products Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Frederick, MD 21702, USA., National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Rockville, MD 20850, USA., Center for Advanced Sensor Technology, Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA. Electronic address: grao@umbc.edu.,
    1. Year: 2023
    2. Date: Apr 11
    3. Epub Date: 2023 04 11
  2. Journal: New Biotechnology
    1. 76
    2. Pages: 13-22
  4. Type of Article: Article
  1. Abstract:

    This study describes the cell-free biomanufacturing of a broad-spectrum antiviral protein, griffithsin (GRFT) such that it can be produced in microgram quantities with consistent purity and potency in less than 24 h. We demonstrate GRFT production using two independent cell-free systems, one plant and one microbial. Griffithsin purity and quality were verified using standard regulatory metrics. Efficacy was demonstrated in vitro against SARS-CoV-2 and HIV-1 and was nearly identical to that of GRFT expressed in vivo. The proposed production process is efficient and can be readily scaled up and deployed wherever a viral pathogen might emerge. The current emergence of viral variants of SARS-CoV-2 has resulted in frequent updating of existing vaccines and loss of efficacy for front-line monoclonal antibody therapies. Proteins such as GRFT with its efficacious and broad virus neutralizing capability provide a compelling pandemic mitigation strategy to promptly suppress viral emergence at the source of an outbreak. Copyright © 2023. Published by Elsevier B.V.

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External Sources

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  2. DOI: 10.1016/j.nbt.2023.04.003
  3. PMID: 37054948
  4. PII : S1871-6784(23)00018-3

Library Notes

  1. Fiscal Year: FY2022-2023
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