Skip NavigationSkip to Content
Return Return to Search Results

The largest HIV-1-infected T cell clones in children on long-term combination antiretroviral therapy contain solo LTRs

  1. Author:
    Botha, Johannes C [ORCID]
    Demirov,Dimiter
    Gordijn, Carli
    Katusiime, Mary Grace
    Bale, Michael J
    Wu,Xiaolin
    Wells, Daria
    Hughes,Stephen
    Cotton, Mark F
    Mellors, John W
    Kearney, Mary F
    van Zyl, Gert U

  2. Author Address

    Stellenbosch University , Cape Town, South Africa., Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research , Frederick, Maryland, USA., HIV Dynamics and Replication Program, National Cancer Institute , Frederick, Maryland, USA., Laboratory of Epigenetics and Immunity, Department of Pathology and Laboratory Medicine, Weill Cornell Medicine , New York, New York, USA., Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania, USA.,
    1. Year: 2023
    2. Date: Aug 02
    3. Epub Date: 2023 08 02
  2. Journal: mBio
    1. Pages: e0111623
  4. Type of Article: Article
  5. Article Number: e0111623
  1. Abstract:

    Combination antiretroviral therapy (cART) suppresses viral replication but does not cure HIV infection because a reservoir of infectious (intact) HIV proviruses persists in long-lived CD4+T cells. However, a large majority (>95%) of HIV-infected cells that persist on effective cART carry defective (non-infectious) proviruses. Defective proviruses consisting of only a single LTR (solo long terminal repeat) are commonly found as endogenous retroviruses in many animal species, but the frequency of solo-LTR HIV proviruses has not been well defined. Here we show that, in five pediatric donors whose viremia was suppressed on cART for at least 5 years, the proviruses in the nine largest clones of HIV-infected cells were solo LTRs. The sizes of five of these clones were assayed longitudinally by integration site-specific quantitative PCR. Minor waxing and waning of the clones was observed, suggesting that these clones are generally stable over time. Our findings show that solo LTRs comprise a large fraction of the proviruses in infected cell clones that persist in children on long-term cART. IMPORTANCE This work highlights that severely deleted HIV-1 proviruses comprise a significant proportion of the proviral landscape and are often overlooked.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1128/mbio.01116-23
  3. PMID: 37530525

Library Notes

  1. Fiscal Year: FY2022-2023
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel