Skip NavigationSkip to Content
Return Return to Search Results

Cell Cycle Regulation of the Human Polo-Like Kinase (Plk) Promoter

  1. Author:
    Uchiumi, T.
    Longo, D. L.
    Ferris, D. K.

  2. Author Address

    Ferris DK NCI INTRAMURAL RES SUPPORT PROGRAM SCI APPLICAT INT CORP FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA NCI INTRAMURAL RES SUPPORT PROGRAM SCI APPLICAT INT CORP FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA NCI LAB LEUKOCYTE BIOL DIV CANC TREATMENT FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA NIA BALTIMORE, MD 21224 USA
    1. Year: 1997
  2. Journal: Journal of Biological Chemistry
    1. 272
    2. 14
    3. Pages: 9166-9174
  4. Type of Article: Article
  1. Abstract:

    Plk (polo-like kinase) is a serine-threonine kinase that appears to function in mitotic control in mammalian cells. We demonstrated previously that PLK mRNA expression is low at the G(1)-S transition, increases during S phase, and is maximally expressed during G(2)-M. In the present study, we have cloned the human PLK gene and analyzed the structure and function of 2 kilobases of its 5'-flanking region. Using synchronized cultures of HeLa cells transfected with PLK promoter/luciferase constructs, we show that the promoter of PLK is activated at S phase and is maximal at G(2)-M phase. Using various PLK promoter/luciferase constructs, we show that three activating regions are located between 35 and 93 base pairs upstream of the transcription initiation site. We identified a repressor element (CDE/CHR) in the region of the transcription start site, and mutations within this element diminished cell cycle regulation of transcription. [References: 28]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at FrederickClose Button

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel