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Chromosomal Changes Observed in Immortalized Human Keratinocytes Transformed By Ionizing Radiation

  1. Author:
    Hukku, B.
    Thraves, P.
    Dritschilo, A.
    Rhim, J. S.

  2. Author Address

    Hukku B CHILDRENS HOSP MICHIGAN DEPT PEDIAT CELL CULTURE LAB 3901 BEAUBIEN BLVD DETROIT, MI 48201 USA WAYNE STATE UNIV SCH MED DETROIT, MI 48201 USA GEORGETOWN UNIV SCH MED DEPT RADIAT MED VINCENT T LOMBARDI CANC RES CTR WASHINGTON, DC USA NCI MOL ONCOL LAB FREDERICK, MD USA
    1. Year: 1997
  2. Journal: Cancer Genetics and Cytogenetics
    1. 93
    2. 2
    3. Pages: 125-139
  4. Type of Article: Article
  1. Abstract:

    Human epithelial cancer cells were induced by concerted action of DNA tumor virus and X-ray radiation. Treatment of nontumorigenic early passage AD12-SV40 immortalized epithelial cells (RHEK-1) at passage 23 with radiation, resulted in further changes in their growth properties. One day old cultures of these RHEK-1 cells were irradiated with graded doses of X-rays (0, 2, 4, 6, and 8 Gy i.e. RHEK-1, RHEK-1/200R, RHEK-1/400R, RHEK-1/600R, and RHEK-1/800R). Morphologic alterations, the ability to grow in soft agar, and to form rapidly-growing squamous cell carcinomas in nude mice were concomitantly acquired properties of the radiation transformed cell lines RHEK-1/200R and RHEK-1/400R. On the basis of commonality in having addition of some extra material in chromosome 11 in the region between q14/q22 in all tumorigenic cell lines RHEK-1/200R and RHEK-1/400R, and deletion of the same region in nontumorigenic irradiated cell lines-RHEK-1/600R and RHEK-1/800R, it is deduced this region may have some important oncogene/s or other gene/s that play an important role in tumorigenesis. When compared to squamous cell carcinoma data, the duplication observed in the present study is also observed in 28 to 38% of head and neck and also in 25% of cases of untreated malignant lesions of oral squamous cell carcinoma. Thus, this study shows the correlation between in vitro induced squamous cell carcinoma to in vivo tumors. (C) Elsevier Science Inc., 1997. [References: 60]

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