Skip NavigationSkip to Content
Return Return to Search Results

Stable isotope dilution high-performance liquid chromatography- electrospray ionization mass spectrometry method for endogenous 2-and 4-hydroxyestrones in human urine

  1. Author:
    Xu, X.
    Ziegler, R. G.
    Waterhouse, D. J.
    Saavedra, J. E.
    Keefer, L. K.

  2. Author Address

    NCI, Off Director, Epidemiol & Biostat Program, Div Canc Epidemiol & Genet, Execut Plaza S,Room 8098,6120 Execut Blvd,MSC 724, Rockville, MD 20852 USA NCI, Off Director, Epidemiol & Biostat Program, Div Canc Epidemiol & Genet, Rockville, MD 20852 USA NCI, Comparat Carcinogenesis Lab, Ctr Canc Res, Frederick, MD 21702 USA SAIC Frederick, Intramural Res Support Program, Frederick, MD 21702 USA Ziegler RG NCI, Off Director, Epidemiol & Biostat Program, Div Canc Epidemiol & Genet, Execut Plaza S,Room 8098,6120 Execut Blvd,MSC 724, Rockville, MD 20852 USA
    1. Year: 2002
  2. Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
    1. 780
    2. 2
    3. Pages: 315-330
  4. Type of Article: Article
  1. Abstract:

    A sensitive, precise and accurate stable isotope dilution high- performance liquid chromatography-electrospray ionization mass spectrometry method has been developed for measuring endogenous 2- and 4-hydroxyestrones, the main catechol estrogens in human urine. Compared to the published methods using gas chromatography-mass spectrometry, this approach simplifies sample preparation and increases the throughput of analysis. The unique part of our method is the use of a simple and rapid derivatization step that forms a hydrazone at the C-17 carbonyl group of catechol estrogens. This derivatization step has greatly enhanced method sensitivity as well as HPLC separability of 2- and 4-hydroxyestrones. Standard curves were linear over a 100-fold calibration range with correlation coefficients for the linear regression curves typically greater than 0.996. The lower limit of quantitation for each catechol estrogen is 1 ng per 10-ml urine sample, with an accuracy of 97-99% and overall precision, including the hydrolysis, extraction and derivatization steps, of 1-3% for samples prepared concurrently and 2-11% for samples prepared in several batches. This method is adequate for measuring the low endogenous levels of catechol estrogens in urine from postmenopausal women. Published by Elsevier Science B.V.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel