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Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants

  1. Author:
    Chen, X.
    Mellon, R. D.
    Yang, L.
    Dong, H. F.
    Oppenheim, J. J.
    Howard, O. M. Z.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Bldg 560,Rm 31-19, Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Frederick, MD 21702 USA NCI, SAIC Frederick, Intramural Res Support Program, Frederick, MD 21702 USA Howard OMZ NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Bldg 560,Rm 31-19, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Biochemical Pharmacology
    1. 63
    2. 3
    3. Pages: 533-541
  4. Type of Article: Article
  1. Abstract:

    Niuhuang is a commonly used Chinese traditional medicine with immunoregulatory and anti-inflammatory properties. Deoxycholic acid (DCA) is a major active constituent of Niuhuang. The reaction of human leukocytes to chemoattractants is an important part of the host immune response and also plays a crucial role in the development of inflammation. We, therefore, investigated the ill vitro effects of DCA on human monocyte and neutrophil responses to classic chemoattractants [fMet-Leu-Phe (fMLP), complement fraction 5a (C5a)], CC chemokine [monocyte chemoattractant protein-1 (MCP-1/CCL2)], and/or CXC chemokines [stromal cell-derived factor-1 (SDF-1alpha/ CXCL12), interleukin-8 (IL-8/CXCL8)]. The results showed that DCA significantly inhibited fMLP-induced monocyte and neutrophil chemotaxis and calcium mobilization, and also blocked the binding of [H-3]fMLP and anti-formyl peptide receptor (FPR) monoclonal antibodies (mAb) to the cells The inhibitory effects of DCA on calcium mobilization and anti-FPR-mAb binding to the receptor could be abrogated by washing DCA out of the cell suspension, suggesting that DCA blocked fMLP receptors via a steric hindrance mechanism, not via receptor internalization, DCA had no significant inhibitory effects on MCP-1-, SDF- 1alpha-, or C5a-induced monocyte function, or C5a- or IL-8- induced neutrophil function. Taken together, our experimental results suggest that blockade of fMLP receptors may contribute to the anti-inflammatory effects of traditional medicine containing DCA. (C) 2002 Elsevier Science Inc. All rights reserved.

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