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Comparative analysis of HIV-1 viral load assays on subtype quantification: Bayer Versant HIV-1 RNA 3.0 versus Roche amplicor HIV-1 monitor version 1.5

  1. Author:
    Elbeik, T.
    Alvord, W. G.
    Trichavaroj, T.
    de Souza, M.
    Dewar, R.
    Brown, A.
    Chernoff, D.
    Michael, N. L.
    Nassos, P.
    Hadley, K.

  2. Author Address

    San Francisco Gen Hosp, Dept Lab Med, 1001 Potrero Ave,NH,Room 2M35, San Francisco, CA 94110 USA Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA San Francisco Gen Hosp, Clin Labs, San Francisco, CA 94110 USA NCI, Data Management Serv, Frederick Canc Res & Dev Ctr, Frederick, MD USA USA, Med Component, Armed Forces Res Inst Med Sci, Bangkok 10400, Thailand Henry M Jackson Fdn, Bangkok, Thailand SAIC Frederick Inc, Frederick, MD USA Elan Pharmaceut, San Francisco, CA USA Walter Reed Army Inst Res, Div Retrovirol, Dept Mol Diagnost & Pathogenesis, Rockville, MD USA Elbeik T San Francisco Gen Hosp, Dept Lab Med, 1001 Potrero Ave,NH,Room 2M35, San Francisco, CA 94110 USA
    1. Year: 2002
  2. Journal: Journal of acquired immune deficiency syndromes (1999)
    1. 29
    2. 4
    3. Pages: 330-339
  4. Type of Article: Article
  1. Abstract:

    Quantification of HIV-1 subtypes is essential for appropriate clinical management. Whereas viral load assays were initially developed to accurately quantify Subtype B, the recent worldwide spread of non-B subtypes, and the introduction of treatment programs in regions with non-B subtypes have prompted adaptations of these assays. The Bayer Versant HIV-1 RNA 3.0 Assay (branched DNA [bDNA] 3.0) and the Roche Amplicor HIV-1 Monitor version 1.5 (Amplicor 1.5) assays are reported to quantify all subtypes in group M however, evaluation of performance characteristics remains limited. In this study, We evaluated the accuracy and reliability of bDNA 3.0 and Amplicor 1.5 on multiple serially diluted viral isolates from HIV-1 group M, subtypes A through F. Testing was conducted on both assay systems in two independent laboratories. Comparative pansubtype quantification from regression analysis showed that quantification by bDNA 3.0 was approximately 0.3 log-fold lower than that by Amplicor 1.5. Comparative pansubtype accuracy analysis showed data points more closely distributed about their respective regression lines and thus showing greater reliability by bDNA 3.0 than by Amplicor 1.5.

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