Skip NavigationSkip to Content
Return Return to Search Results

Mitochondrial damage and DNA depletion in cord blood and umbilical cord from infants exposed in utero to Combivir

  1. Author:
    Divi, R. L.
    Walker, V. E.
    Wade, N. A.
    Nagashima, K.
    Seilkopf, S. K.
    Adams, M. E.
    Nesel, C. J.
    O'Neill, J. P.
    Abrams, E. J.
    Poirier, M. C.

  2. Author Address

    Poirier, MC, NCI, CDI Sect, NIH, Bldg 37,Rm 4032,37 Convent Dr,MSC-4255, Bethesda, MD 20892 USA NCI, CDI Sect, NIH, Bethesda, MD 20892 USA. Lovelace Resp Res Inst, Albuquerque, NM USA. Albany Med Ctr, Childrens Hosp, Dept Pediat, Albany, NY USA. New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA. NCI, SAIC, Frederick, MD 21701 USA. SKS Consulting Serv, Siler City, NC USA. Westat Corp, Rockville, MD USA. Univ Vermont, Genet Lab, Burlington, VT USA. Columbia Univ, Coll Phys & Surg, Dept Pediat, New York, NY USA. Columbia Univ Coll Phys & Surg, Harlem Hosp Ctr, New York, NY 10032 USA.
    1. Year: 2004
  2. Journal: Aids
    1. 18
    2. 7
    3. Pages: 1013-1021
  4. Type of Article: Article
  1. Abstract:

    Objective: Although most uninfected infants born to women infected with HIV-1 show no clinical evidence of mitochondrial compromise, mitochondrial dysfunction has been reported in children born to women receiving zidovudine and/or lamivudine during pregnancy. In this pilot study we examined mitochondrial integrity in HIV-1-uninfected infants born to HIV-1-infected women receiving Combivir during pregnancy.Design: Samples of umbilical cord and cord blood were obtained from HIV-1-uninfected infants born to either HIV-1-infected women receiving Combivir therapy during pregnancy (n = 10) or HIV-1-uninfected women (n = 9).Methods: Mitochondrial morphological integrity was examined in umbilical cords (n = 16) by electron microscopy and mtDNA quantity was determined in DNA from cord blood (n = 18) and umbilical cord (n = 18) by PCR-chemiluminescence immunoassay detection.Results: In umbilical cords from six of nine infants born to HIV-1-infected mothers taking Combivir moderate to severe mitochondrial morphological damage was observed (P = 0.011), while none of seven unexposed infants showed similar damage. Compared to unexposed infants, statistically significant mtDNA depletion was observed in umbilical cord (P = 0.006) and cord blood (P = 0.003) from drug-exposed infants.Conclusions: A cohort of HIV-1-uninfected Combivir-exposed infants with no clinical symptoms showed morphological and molecular evidence of mitochondrial damage. (C) 2004 Lippincott Williams Wilkins

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel