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Expression and evaluation of RING finger proteins

  1. Author:
    Yang, Y. L.
    Lorick, K. L.
    Jensen, J. P.
    Weissman, A. M.

  2. Author Address

    NCI, Lab Prot Dynam & Signaling, Canc Res Ctr, Frederick, MD 21702 USA Yang, YL, NCI, Lab Prot Dynam & Signaling, Canc Res Ctr, Frederick, MD 21702 USA
    1. Year: 2005
  2. Book Title: Methods in Enzymology
  3. Series Title: Methods in enzymology
    1. 398
    2. Pages: 103-112
  5. Type of Work: Review
  1. Abstract:

    RING finger proteins represent the largest class of potential ubiquitin ligases. This chapter describes methods used to express and assess the activity of proteins containing RING fingers based on our experience with a number of different family members. In addition to general protocols for assessing activity, specific protocols are provided for evaluating the ubiquitylation of p53 by the RING finger E3 Hdm2/Mdm2. Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation

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External Sources

  1. View record in Web of Science®
  2. WOS: 000233007300010

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