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Carbohydrate array analysis of anti-Tn antibodies and lectins reveals unexpected specificities: Implications for diagnostic and vaccine development

  1. Author:
    Manimala, J. C.
    Li, Z. T.
    Jain, A.
    VedBrat, S.
    Gildersleeve, J. C.

  2. Author Address

    NCI, Ctr Canc Res, Med Chem Lab, Frederick, MD 21702 USA. KamTek Inc, Gaithersburg, MD 20879 USA Gildersleeve, JC, NCI, Ctr Canc Res, Med Chem Lab, 376 Boyles St,Bldg 376,Room 109, Frederick, MD 21702 USA
    1. Year: 2005
    2. Date: DEC
  2. Journal: Chembiochem
    1. 6
    2. 12
    3. Pages: 2229-2241
  4. Type of Article: Article
  1. Abstract:

    The Tn antigen is a carbohydrate antigen expressed in most carcinomas, during embryogenesis, on pathogenic parasites, and on HIV. It has been evaluated extensively as a potential diagnostic marker and several Tn-based vaccines are in clinical trials. Based on discrepancies in the literature regarding Tn expression, we began to question whether antibodies and lectins used routinely to, detect the Tn antigen were providing accurate information. To investigate this possibility, a carbohydrate microarray and a highly sensitive assay were developed and three frequently used Tn receptors (HBTn1, Bric111, and VVL-B4) were evaluated. Carbohydrate-array analysis revealed unexpected cross-reactivity with other human carbohydrate epitopes. WL-B4 bound the Tn antigen, GalNAc alpha l-6Gal, and GalNAc alpha 1-3Gal. Bric111 bound the Tn antigen, blood group A GalNAc alpha 1-6Gal, and GalNAc alpha 1-3Gal. HBTn1 showed the best selectivity but still displayed moderate binding to blood group A. Implications for the development of Tn-based diagnostics and vaccines are discussed

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  1. View record in Web of Science®
  2. WOS: 000233916600018

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