Skip NavigationSkip to Content
Return Return to Search Results

Altered expression of neuronal nitric oxide synthase in the duodenum longitudinal muscle-myenteric plexus of obesity induced diabetes mouse: Implications on enteric neurodegeneration

  1. Author:
    Surendran, S.
    Kondapaka, S. B.

  2. Author Address

    Univ Texas, Med Branch, Dept Internal Med, Galveston, TX 77555 USA. NCI, Screening Technol Branch, NIH, Frederick, MD 21702 USA Surendran, S, Univ Texas, Med Branch, Dept Internal Med, Galveston, TX 77555 USA
    1. Year: 2005
    2. Date: DEC 16
  2. Journal: Biochemical and Biophysical Research Communications
    1. 338
    2. 2
    3. Pages: 919-922
  4. Type of Article: Article
  1. Abstract:

    Type 2 diabetes caused by obesity shows autonomic neuropathy. Molecular mechanism involved in enteric neurodegeneration is not clear. Neuronal nitric oxide synthase (nNOS) is one of the important agents involved in gastrointestinal function. Therefore, expression of nNOS in the duodenum LM-MP of type 2 diabetes model mouse was studied. Real time RT-PCR analysis showed reduction in nNOS expression in male diabetic LM-MP compared to male control. In contrast, female diabetic LM-MP had high level of nNOS mRNA compared to female control. Western blot determination of LM-MP showed reduction in nNOS protein in male diabetic LM-MP and high level of nNOS in female diabetic LM-MP compared to their respective controls. Expression of nNOS observed by Western blot was further confirmed by nNOS immunostaining of the mouse duodenum. TUNEL staining of mouse duodenum showed apoptosis in male diabetic enteric neurons. These studies suggest that nNOS expression in LM-MP varies with gender during early stage of type 2 diabetes. In addition, reduced expression of nNOS is likely to contribute to apoptosis seen in the enteric neurons of male type 2 diabetic mice. (c) 2005 Elsevier Inc. All rights reserved

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.bbrc.2005.10.039
  3. View record in Web of Science®
  4. WOS: 000233451100033

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel