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Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction

  1. Author:
    McAvin, J. C.
    Bowles, D. E.
    Swaby, J. A.
    Blount, K. W.
    Blow, J. A.
    Quintana, M.
    Hickman, J. R.
    Atchley, D. H.
    Niemeyer, D. M.

  2. Author Address

    USAF, Inst Environm Safety & Occupat Hlth Anal, Epidemiol Surveillance Div, San Antonio, TX 78235 USA. USAF, Force Protect Battlelab, Lackland Air Force Base, San Antonio, TX 78236 USA. USAF, Sch Aerosp Med, Brooks Air Force Base, San Antonio, TX 78235 USA. USA, Med Res Inst Infect Dis, Div Virol, Frederick, MD 21702 USA. USA, Ctr Hlth Promot & Prevent Med W, Div Environm Sci, Ft Lewis, WA 98433 USA. USAF, Off Surg Gen, Baolling Air Force Base, Washington, DC 20332 USA. Joint Program ExecutChem Biol Def, Falls Church, VA 22041 USA.;McAvin, JC, USAF, Inst Environm Safety & Occupat Hlth Anal, Epidemiol Surveillance Div, San Antonio, TX 78235 USA.
    1. Year: 2005
    2. Date: Dec
  2. Journal: Military Medicine
    1. 170
    2. 12
    3. Pages: 1060-1065
  4. Type of Article: Article
  5. ISSN: 0026-4075
  1. Abstract:

    An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles stephensi, and Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demonstrated 100% concordance in both in vitro sensitivity (six of six samples) and specificity (10 of 10 samples). A single adult A. aegypti was identified in a pool of 100 non-A. aegypti mosquitoes. The limit of detection of A. aegypti egg pools was five individual eggs. Field testing was conducted in central Honduras, An A. aegypti and Culex spp. panel of individual and mixed pools (n = 30) of adult mosquitoes, pupae, and larvae demonstrated 100% concordance in sensitivity (22 of 22 samples) and 97% concordance in specificity (29 of 30 samples), with one false-positive result. Field testing of an A. aegypti and Culex spp. blind panel (n = 16) consisting of individual and mixed pools of adult mosquitoes, pupae, and larvae demonstrated 90% concordance in sensitivity (nine of 10 samples) and 88% concordance in specificity (14 of 16 samples).

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External Sources

  1. View record in Web of Science®
  2. WOS: 000235832200017

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